Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Cytotoxicity of Phenolic Compounds Isolated from Seeds of Safflower (Carthamus tinctorius L.) on Cancer Cell Lines

Bae, Song-Ja;Shim, Sun-Mi;Park, Yun-Ja;Lee, Jun-Young;Chang, Eun-Ju;Choi, Sang-Won

  • Published : 20020400
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Abstract

The methanolic extract of roasted safflower seeds exhibited moderate cytotoxicity against three cancer cell lines, HepG2, MCF-7, and Hela, in a dose-dependent manner, as measured by MIT assay. The methanolic extract was further partitioned with n-hexane, ethyl acetate and n-butanol successively. The ethyl acetate fraction exhibited potent cytotoxicities against the cancer cell lines. NFeruloylserotonin (Sl), N-(p-coumaroyl)serotonin (S2), matairesinol (L1), 8'-hydroxyarctigenin (L2), luteolin (F1), and acacetin (F2) were isolated from the ethyl acetate extract, and their chemical structures were identified by UV, IR, NMR, and MS spectroscopic methods. Six phenolic compounds possessed comparable cytotoxicity against three cancer cells. In particular, F1 and F2 had the most potent cytotoxicity with $IC_{50}$ values of 51.8 and $62.1{\mu}g/mL$ for Hela cell, 33.6 and $37.7{\mu}g/mL$ for MCF-7 cell and 47.3 and $56.6{\mu}g/mL}$ for HepG2 cell, respectively. In addition, L1, L2, Sl, and S2 also showed strong cytotoxicity, although activities of serotonins were weaker than those of lignans. On normal human liver cell (WRL68) at a high concentration of $100{\mu}g/mL}$ two serotonins and lignans did not show any cytotoxicity, and two flavonoids exhibited only about 50% cytotoxicity. These results suggest that phenolic compounds in the safflower seeds may be useful as potential cancer chemopreventive agents.

Keywords

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