Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Morphological and Physiological Characteristics of Emblings Derived from Somatic Embryos , Seedlings , and Mature Trees in Larix leptolepis

낙엽송(Larix leptolepis) 체세포배 유래 식물체와 종자 실생묘 및 성숙목간의 형태, 생리적 특징

Kim, Yong-Wook;Kim, Joon-Chul;Youn, Yang;Moon, Heung-Gyu;Lee, Jae-Soon
김용욱;김준철;윤양;문흥규;이재순

  • Published : 2002.03.31
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Abstract

The 32-year-o1d trees, 32-year old seedlings and emblings which regenerated from somatic embryos werecompared to the foliar characteristics such as size of the needles (length, width, dry weight and surfacearea), stomata shapes (length, width and density) and chlorophyll contents (chlorophyll a, b and totalchlorophyll) in Larix leptolepis. The seedlings and emblings were similar in terms of length (35.6+-3.6,36.9+-3.9mm), width (1.3+-0.03, 1.4+-0.lmm), dry weight (2.26+-0.07, 3.78+-0.12mg) and surface area (0.36+-0.01, 0.42+-0.03cm2) of needle. Seedlings and emblings showed similar size of the length, width ofstomata except 3-year old seedlings with the lowest width (10.2+-0.12/um). However, the density ofstomata was variable among the plants. In comparison of chlorophyll content in the needles, the chlorophylla and b were similar in all examined plants, and but 32-year old trees showed the highest content ofchlorophyll a (990+-6.3ug/g) and b (930+-6ug/g), respectively. In case of total chlorophyll content, allplants showed similar pattern whereas the 32-year old trees was the highest content (2160+-12ug/g) amongthe trees.

낙엽송의 32년생 성목, 3년생 실생묘, 체세포배로부터 유래된 식물체간에 침엽의 형태(길이, 폭, 건중량 및 표면적), 기공의 형태(길이, 폭 및 밀도), 그리고 엽록소 함량(엽록소 a, b 및 총 엽록소량)을비교하였다. 침엽의 길이(35.6+-3.6, 36.9+-3.9mm), 폭(1.3+-0.03, 1.4+-0.1mm), 건중량(2.26+-0.07,3.78+-0.12mg) 및 표면적(0.36+-0.01, 0.42+-0.032cm2)은 실생묘와 체세포배 유래 식물체간에 비슷한수치를 보였다. 그리고 기공의 길이와 폭 비교에서는 가장 작은 폭인 10.2+-0.12um를 보인 3년생의 실생표를 제외하곤 거의 비슷하였다. 그러나 기공 밀도에서는 식물체간에 다양한 밀도 수치를 보였다. 성목, 실생묘. 체세포배 유래 식물체간의 엽록소 함량 비교에서 엽록소 a와 b는 모든 식물체에서 비슷한수치를 보였으나 32년생 성목에서 가장 높은 엽록소 a(990+-6.3ug/g) 및 b(930+-6ug/g)를 나타냈다. 총엽록소 함량도 시료에서 비슷하게 나타났지만, 특히 32년생 성목에서 가장 높은 값(2160+-12ug/g)을 보였다.

Keywords

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