Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Micropropagation of Kalopanax Pictus Nakai via Axillary Bud Cultures

음나무의 액아배양에 의한 기내번식

Moon, Heung-Kyu;Kim, Se-Hyun;Kim, Bong-Kyu
문흥규;김세현;김봉규

  • Published : 20021200
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Abstract

An efficient shoot proliferation system has been tried from axillary bud cultures of 2-year-old Kalopanax pictus. Axillary buds were cultured on MS and WPM medium in combination of BA or kinetin to induce multiple shoots. Although every explant showed bud burst from leaf primordia within a week. multiple shoots did not develop from initial cultures. In general, leaf growth was better on MS medium than on WPM medium. However, petioles were well developed on WPM medium. Shoot tip necrosis and hyperhydricity were appeared in the serial cultures on both media. This malformation symptom was severe on MS medium compared to WPM medium. However. the necrotic rate was shown irreversibly on the media supplemented with the increasing concentration of cytokinin. Even though shoot proliferation was successful by the extension of 4 -week-subculture on WPM medium containing 0.5mg/L kinetin. shooting capacity was relatively low with the production of only 2 to 3 shoots per explant. In vitro rooting rate was extremely low with about 10% by the application of naphtaleneacetic acid (NAA). Nevertheless. ~ vitro rooting was drastically increased upto 60% on sterilized artificial soil mixture. In addition the rooted cuttings showed better root growth in greenhouse condition and survived more than 90%. Our results provide the possibility of shoot proliferation via axillary bud cultures of K. pictus. Furthermore, it suggests that the more optimized cultural system should be necessary 10 produce multiple shoots of the species via tissue culture.

2년생 음나무의 액아배양을 통한 효과적인 기내중식의 가능성을 시첩하였다. 액아를 절편으로 MS 및 WPM 배지에 BA 및 kinetin을 농도별로 처리하여 줄기유도를 시칩하였다. 초대배양 후 1주내에 배지 및 싸이토키닌의 처리에 관계없이 100% 잎의 발생이 이루어 졌으나 다경유도는 이루어지지 못했다. 대체적으로 잎의 생장은 MS 배지에서, 엽병의 생장은 WPM 배지에서 양호하였다. 배양과정에서 잎이나 줄기의 선단에 괴저나 과수화가 흔히 관찰되었고, MS 배지에서 심한 경향이었다. 그러나 괴저율은 두 배지에서 싸이토키닌의 처리농도가 높아질수록 감소하였다. 한편 다경 유도는 0.5mg/L kinetin 처리된 WPM 배지에서 가능하였으나 절편 당 2-3개로 증식율은 저조하였다. 증식된 줄기의 기내발근율은 NAA 처리시 10% 정도로 저조하였으나, 멸균된 인공상토에 직접 기외삽목 할 경우 60% 발근되고 뿌리의 발달도 양호하였다. 발근묘는 온실에서 환경순화 후 90% 이상 활착되었다. 이상의 결과는 음나무의 액아배양으로 기내번식의 가능설을 보여 주었으나 대량생산을 위해서는 배양 적정화를 통한 증식율 제고가 더욱 필요한 것으로 나타났다.

Keywords

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