Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Hijikia fusifome Ethanol Extract on Antioxidative Enzymes in Ethanol-induced Hepatotoxicity of Rat Liver

톳 에탄올 추출물이 알코올을 투여한 흰쥐의 항산화효소활성에 미치는 영향

  • 고무석 (전남대학교 가정교육과) ;
  • 신길만 (순천대학교 조리과학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2002.02.01
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Abstract

This study was designed to investigate the effects of Hijikia fusiforme (Harvey) Okamura ethanol extract on the ethanol-induced hepatotoxicity of rat administered orally experimental diets for 6 weeks. Sprague-Dawley rats weighing about 100 g were divided into 4 groups; normal group (NOR), ethanol (35% ethanol 10 mL/kg b.w/day) treated group (CON), ethanol and Hijikia fusiforme ethanol extract 200 mg/kg (HE1) and 400 mg/kg (HE2) concomitantly treated group, respectively. Each group was examined for the growth rate, feed efficiency ratio (FER), activities of antioxidative enzymes and contents of TBARS and glutathione. Hijikia fusiforme ethanol extract showed increasing effects of the growth rate by 43%, and FER was gradually increased by Hijikia fusiforme ethanol extract treatment, compard with ethanol treatment. Ethanol elevated the activities of superoxide dismutase, catalase and glutathione peroxidase of rat liver markedly as compared to normal group, but those activities were significantly decreased in Hijikia fusiforme ethanol extract treatment by 56%, 38% and 25%, respectively. Xanthine oxidase activity elevated by ethanol was not affected by Hijikia fusiforme ethanol extract. The content of TBARS increased by ethanol treatment was signigicantly decreased in HE2, and the glutathione content depleted by ethanol treatment was increased by Hijikia fusiforme ethanol extract administration adjacent to normal level. These results suggest that Hijikia fusiforme ethanol extract is believed to be a possible protective effect for the ethanol-induced hepatotoxicity of rat liver.

톳 에탄올 추출물이 알코올을 투여한 흰쥐의 간조직 손상에 미치는 영향을 알아보기 위해 정상군, 알코올 투여군(35% ethanol, 10mL/kg B.W./day) 알코올 및 톳 에탄올 추출물 200mg/kg 및 400mg/kg 병합투여군의 4군으로 나누어 6간 사육 후 체중 증가율, 식이효율, 혈청중 ALT 및 ALP활성, 간조직의 손상억제효과를 검토하기 위해 SOD, catalase, XO 및 GSH-Px효소 활성을 측정하고 지질과산화물인 TBARS 와 GSH함량을 측정한 결과는 다음과 같다. 1) 체중증가율은 알코올 투여군이 정상군에 비하여 약 38%가 감소되었으나 알코올과 톳 에탄올 추출물(kg당 200mg 및 400mg)을 병합투여하여 알코올에 의해 둔화된 체중증가율이 정상군에 근접하도록 회복되었으며, 식이효율은 알코올 투여군이 정상군에 비하여 약 50%가 감소되었으나 톳 에탄올 추출물과 알코올의 병합투여로 증가되었다. 2) 간 손상지표 중의 하나인 혈청 ALT 및 ASP활성의 경우, 알코올 투여로 정상군에 비하여 160%정도 유의적으로 증가되었으나, 알코올과 톳 에탄올 추출물의 병합 투여로 알코올 투여군에 비하여 각각 63% 및 47%가 감소되었다. 3) 톳 에탄올 추출물은 알코올에 의해 증가된 세포질내의 XO활성을 감소시키지 못했으나 SOD, catalase 및 GSH-Px활성은 알코올 투여군에 비하여 각 56%, 38% 및 28%가 감소되었다. 4) 알코올만을 투여한 군의 TBARS함량은 정상군에 비하여 약 129%이상 증가되었으나, 알코올과 톳 에탄올 추출물 병합 투여로 알코올만을 투여한 군에 비하여 각각 34%, 56%의 감소를 나타냈는데 특히 400mg 투여가 더 많은 감소 효과를 보였다. 5) 알코올 투여로 간조직중의 GSH함량은 정상군에 비해 약 38%가 감소되었으나, 알코올과 톳 에탄올 추출물 병합 투여로 알코올 투여군에 비하여 각각 33% 및 66%가 증가되었다. 특히 400 mg 투여군의 경우 GSH함량 상승 효과가 우수하였다.

Keywords

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