Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Total Microbial Biomass Measured by ATP in Three Marine Sedimentary Environments

아데노신 3인산(ATP; Adenosine-5′ triphosphate)을 이용한 심해저 및 연안퇴적토의 총 미생물 생체량 측정

  • 현정호 (심해저자원 연구센터) ;
  • 김경홍 (심해저자원 연구센터) ;
  • 권개경 (한국해양연구원 미생물 연구실) ;
  • 이정현 (한국해양연구원 미생물 연구실) ;
  • 이홍금 (한국해양연구원 미생물 연구실) ;
  • 김상진 (한국해양연구원 미생물 연구실) ;
  • 김기현 (심해저자원 연구센터)
  • Published : 2002.06.01
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Abstract

ATP concentrations far estimating total microbial biomass in the sediment were measured in three different marine sedimentary environments. ATP concentrations were highest in the surface sediment and decreased with increasing sediment depth and distance from the land. The results indicated that the benthic microbial biomass is primarily controlled by nutrient inputs from the overlying water column. Because of the longer residence time and adsorption to the sediment, the variations in organic carbon (OC) contents with sites and depths were not as distinct as that of ATP, and the correlation between OC and ATP was not significant in the coastal sediments. No significant correlation between OC and ATP in the coastal sediments also suggested that microbial biomass in the labile organic-enriched coastal sediment is suppressed by the grazing of higher trophic level such as meiofauna. Overall regional and vertical distribution of ATP indicated that h\`w can be a relevant tool for measuring total microbial biomass in various marine sedimentary environments.

심해와 연안의 서로 다른 해양퇴적토 환경에서 ATP농도를 이용한 총 미생물 생체량을 측정하였다. 표층 ATP의 분포는 연안역에서 가장 높았으며, 육지로부터 멀어질수록 감소하였다. 또한 수직적으로는 깊이에 따라 급격히 감소하는 양상을 나타내, 저층의 생태계가 수층으로 부터의 영양원 공급에 의해 일차적으로 조절되고 있는 것으로 나타났다. 한편, 유기물은 퇴적물로의 흡착으로 인해 환경 내 체류시간이 길어짐으로 인해, 유기탄소의 함량 변화가 ATP에 비해 급격히 일어나지 않았으며, 특히, 연안퇴적토의 경우 유기탄소의 함량 변화에 비해 ATP의 농도변화가 일정하게 나타나 두 변수간의 유의성 이 없는 것으로 나타났다. 이러한 결과는 분해가능한 유기물의 공급이 많은 연안퇴적토의 경우, 영양원에 의한 조절보다는 상위영양단계의 포식활동이 미생물의 생체량을 최종적으로 조절하는 요인으로 작용하기 때문 인 것으로 사료된다. 지역분포 특성 및 수직적 분포 특성으로부터 ATP는 서로 다른 저서 생태환경의 생체량 분석을 위한 일차적 인 도구로 유용하게 쓰일 수 있는 것으로 나타났다.

Keywords

References

  1. 한국의 갯벌(중) 우리나라 갯벌의 규모와 간척 고철환;고철환(편)
  2. '99 심해저 광물자원 탐사 보고서 해양수산부
  3. 서태평양 종합대양연구(Ⅰ) 한국해양연구원
  4. Microbiol. Rev. v.59 Phylogenetic identification and in situ detection of individual microbial cells without cultivation Amann. R.I.;W. Ludwig;K.-H. Schleifer
  5. Marine Analytical Chemistry Standards Program, Division of Chemistry Marine sediment reference materials for trace metals and other consituents Berman, S.
  6. Native aquatic bacteria: enumeration, activity, and ecology The plate count in aquatic microbial ecology Buck, J.D.;J.W. Costerton(ed.);R.R. Colwell(ed.)
  7. Soil. Sci. v.119 Distribution of microbial adenosine triphosphate in salt marsh sediments at Sapelo Island, Georgia Christian, R.R.;K. Bancroft;W.J. Wiebe https://doi.org/10.1097/00010694-197501000-00013
  8. Native aquatic bacteria: enumeration, activity, and ecology Direct epifluorescence enumeration of native aquatic bacteria: uses, limitations, and comparative accuracy Daley, R.J.;J.W. Costerton(ed.);R.R. Colwell(ed.)
  9. Handbook of methods in aquatic microbial ecology Analysis of microbial lipids to determine biomass and detect the response of sedimentary microorganism to disturbance Dobbs, F.C.;R.H. Findlay;P.F. Kemp(ed.);B.F. Sherr(ed.);J.J. Cole(ed.)
  10. Marine mineral resources Earney, F.C.F.
  11. Mar. Ecol. Prog. Ser. v.117 Enumeration of sandy sediment bacteria: search for optimal protocol Epstein, S.S.;J. Rossel https://doi.org/10.3354/meps117289
  12. Bacterial biogeochemistry(2nd ed.) Fenchel, T.;G.M. King;T.H. Blackburn
  13. Estuar. Res. v.1 Microbial ATP and organic carbon in sediments of the Newport River Estuary, North Carolina Ferguson, R.L.;M.B. Murdoch
  14. Geochim. Cosmochin. Acta. v.53 Dissolved free amino acids in hydrothermal vent habitats of the Guaymas Basin Haberstroh, P.R.;D.M. Karl https://doi.org/10.1016/0016-7037(89)90170-1
  15. [The Sea] J. Kor. Soc. Oceanogr. v.3 Distibution of ATP in the deep-sea sediment in the KODOS 97-2 area, northeast equatorial Pacific Ocean Hyun, J.-H.;K.-H. Kim;S.-B. Chi;J.-W. Moon
  16. Bacteria in nature Determination of in situ microbial biomass, viability, metabolism, and growth Karl, D.M.;J.S. Poindexter(ed.);E.R. Leadbetter(ed.)
  17. Handbook of methods in aquatic microbial ecology Total microbial biomass estimation derived from the measurement of particulate adenosine-5'-triphosphate Karl, D.M.;P.F. Kemp(ed.);B.F. Sherr(ed.);E.B. Sherr(ed.);J.J. Cole(ed.)
  18. Deep-Sea Res. v.23 Adenosine triphosphate in the North Atlantic Ocean and its relationship to the oxygen minimum Karl, D.M.;P.A LaRock;J.W. Morse;W. Sturges
  19. Science v.207 Deep-sea primary production at the Galapagos hydrothermal vents Karl, D.M.;C.O. Wirsen;J.W. Jannasch https://doi.org/10.1126/science.207.4437.1345
  20. Mar. Biol. Lett. v.5 Thermophilic microbial activity in samples from deep-sea hydrothermal vents Karl, D.M.;D.J. Burns;K. Orrett;H.W. Jannasch
  21. Nature v.370 Sorptive preservation of lablile organic matter in marine sediment Keil. R.G.;D.B. Montlucon;F.G. Prahl;H.I Hedge https://doi.org/10.1038/370549a0
  22. Microbiol. Rev. v.58 Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present Kepner, Jr. R.L.;J.R. Pratt
  23. Chem. Geol. v.114 Relationship between mineral surfaces and organic carbon concentrations in soils and sediments Mayer, L.M. https://doi.org/10.1016/0009-2541(94)90063-9
  24. Mar. Ecol. Prog. Ser. v.18 In situ measurement of meiobenthic grazing rates on sediment bacteria and edaphic diatoms Montagna, P.A. https://doi.org/10.3354/meps018119
  25. Appl. Environ. Mocroiol. v.53 Micobial growth rates and biomass production in a marine sediment: evidence for a very active but mostly nongrowing community Novitsky, J.A.
  26. Descriptive physical oceanography: an introduction(4th ed.) Pickard, G.L.;W.J. Emery
  27. Neth. J. Sea Res. v.13 Food and food uptake in Arenicola marina Rijken, M. https://doi.org/10.1016/0077-7579(79)90014-0
  28. Deep-Sea Res. v.40 The effect of pressure on leucine and thymidine incorporation by free-living bacteria attached to sinking oceanic particles Turley, C.M.
  29. Oecologia v.40 Determination of the sedimentary microbial biomass by extractible lipid phosphate White, D.C.;W.M. Davis;J.S. Nickles;J.D. King;R.I. Bobbie https://doi.org/10.1007/BF00388810