Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effect of Prunus Mume Extracts on the Growth of HepG2 and HeLa Cell Lines

간암 및 자궁암 세포주 증식에 미치는 오매 추출물의 영향

  • 배지현 (계명대학교 식품영양학과) ;
  • 정승은 (계명대학교 식품영양학과)
  • Published : 2002.05.01
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Abstract

This study was undertaken to investigate the inhibitory effect of prunus fume extracts on the growth of Hep G2 and HeLa cells. Prunus mums was extracted using the following solvents hexane, chloroform, ethylacetate, methanol, and hot water. The effect on the growth of each cancer cell line was examined by MTT (3-[4, 5-dimethylthiaeol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, cytotoxicity testing, and microscopic observation. The ethylacetate extracts of Prunus muse at the concentration of 250 $\mu\textrm{g}$/ml exhibited the greatest inhibitory effect on the growth of Hep G2 in the MW assay. In cytotoxicity testing, the treatment of the Hep G2 cells with ethylacetate extracts (1000 $\mu\textrm{g}$/ml for 72 hrs) destroyed 75% of the cells, and morphological changes were also observed. futhermore, the hexane extracts of Prunes muse at the concentration of 250 $\mu\textrm{g}$/ml exhibited the greatest inhibitory effect on the growth of HeLa cells in the MTT assay. The treatment of the HeLa cells with the hexane extracts (1000 $\mu\textrm{g}$/ml for 72 hrs) resulted in the destruction of 68% of the cells. Fibroblasts were not affected by either ethylacetate or hexane extracts of prunus muse.

본 연구에서는 예로부터 민간과 한방에서 널리 이용되어 온 오매를 이용하여 오매 추출물이 간암 및 자궁암 세포주 증식에 미치는 효과를 알아보았다. 오매를 hexane. chloroform, ethylacetate, methanol 및 열수로 순차 추출하여 분획물을 농도별로 조제하였으며, MTT assay, cytotoxicity test 및 현미경 관찰 등을 통하여 암세포의 성장 억제 효과를 알아보았다. Hep G2의 경우 오매의 ethylacetate추출물이 가장 큰 성장 저해 효과를 나타내었으며 MTT assay결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다. 또한 cytotoxicity test결과 오매의 ethylacetate 추출물 1000$\mu\textrm{g}$/ml농도에서 72시간 배양시 75%의 세포 파괴율을 나타내었으며 현미경 관찰 결과 세포의 모양이 변화되고 죽은 세포가 배양액위에 떠있음을 관찰할 수 있었다. HeLa cell에서는 오매의 hexane 추출물이 가장 큰 저해 효과를 나타내었으며 MTT assay 결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다 또한 cytotoxicity test 결과 오매의 hexane추출물 1000 $\mu\textrm{g}$/ml농도에서 72시간 배양시 HeLa cell의 68%가 파괴되었으며 현미경 관찰 결과 세포 모양의 형태학적 변화가 일어났다. 정상세포주인 fibroblast에는 오매의 ethylacetate 및 hexane 추출물이 아무런 유의적 영향을 미치지 않았으며 현미경 관찰 시에도 변화가 일어나지 않았다

Keywords

References

  1. J. of the Korean Society of Health Statistics v.23 A Study on the related factors of Korean cancer-outbreaks Kim SS
  2. J Natl Cancer Inst v.66 The causes of cancer Doll R;Peto R
  3. J Natl Cancer Inst v.58 Contribution of the environment to cancer incidence;an epidemiologic exercise Wynder EL;Gori G https://doi.org/10.1093/jnci/58.4.825
  4. The molecular basis of cancer Chemical carcinogenesis Phillips DH
  5. Cancer Res v.52 Food -derived mutagens and carcinogens Wakabayashi K;Nagao M;Esumi H;Sugimura T
  6. Cancer Res v.52 Inhibition of carcinogenesis by minor dietary constituents Wattenberg LW
  7. J Korean Soc Food Nutr v.20 Naturally-occuring novel anticarcinogens: Conjugated dienoic derivatices of linoleic acid (CLA) Ha YL;Michael WP
  8. Chem Pharm Bull v.29 Structural examination of a water soluble antitumor polysaccharide of Ganoderma lucidum Miyazaki T;Nishijima M https://doi.org/10.1248/cpb.29.3611
  9. Health Foods form Herbs Jee HJ
  10. M S Thesis, Kyungnam Univ. Effect of Maesil extracts ingestion on blood lactate density and serum lipid components Youn MS
  11. J Korean Soc Food Nutr v.19 Effect of Prunus mume extracts on the gastric secretion in rats and carbon tetrachloride induced liver damage of rabbits Sheo HJ;Lee MY;Chung DL
  12. J Korean Soc Food Nutr v.16 Effect of Prunus mume extracts on experimentally Alloxan induced diabetes in rabbits Sheo HJ;Ko EY;Lee MY
  13. J East Asian Diet Life v.9 no.2 Effect of Prunus mume extract containing beverages on the proliferation of food-borne pathogens Bae JH;Kim KJ
  14. Cell culture Methods in enzymology Vol. Ⅷ Jacoby WB;Pastan LH
  15. Cancer Res v.48 Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay Alley MC;Scudiero DA;Monks A;Hursey ML;Czerwinski MJ;Fine DL
  16. J Immunol Methods v.65 Rapid colorimetric assay for cellular growth and survial: Application to proliferation and cytotoxicity assay Mosman T https://doi.org/10.1016/0022-1759(83)90303-4
  17. J Cell Physiol v.123 1,25-dihydroxyvitamin D₃specific regulation of growth, morphology and fibronectin in a human osteosarcoma cell line Franceschi RT;James WM;Zerlauth G https://doi.org/10.1002/jcp.1041230316
  18. Korean J Nutr v.31 Inhibitory effect of Artemisia princeps Pampan extracts on growth of cancer cell lines Hwang YK;Kim DC;Hwang WI;Han YB
  19. J Korean Soc Food Sci Nutr v.28 Anticancer effect of Doenjang in vitro Sulforhodamine (SRB) Assay Lim SY;Park KY;Rhee SH
  20. Kor J Appl Microbiol Biotechnol v.25 Antitumor effects of extracts obtained from Daedalea dickinsii Yang KH;Yang JH;Ryu BH
  21. Kor J Appl Microbiol Biotechnol v.21 Inhibitory effects of Chinese pepper on the mutagenicity and the growth of MG-63 human osteosarcoma Kim SH;Park KY
  22. Korean J Ginseng Sci v.17 Comparative study on the cytotoxic activities of red ginseng of Korea and China Hwang WI;Sohn JW
  23. Korean J Food Sci Technol v.23 Inhibitory effects of Garlic on the mutagenicity in Salmonella Assay system and on the growth of HT-29 Park KY;Kim SH;Suh MJ;Chung HY
  24. Cancer Res v.55 Vegetable consumption, serum retinol level, and risk of hepatocellular carcinoma Yu MW;Hsieh HH;Pan WH;Yang CS;Chen CJ
  25. Int J Cancer v.42 Risk factors for hepatocellular carcinoma in Northern Italy LaVecchia C;Negri E;Decarli A;D'Avanzo B;Franceschi S https://doi.org/10.1002/ijc.2910420614
  26. Int J Cancer v.43 A case-control study of diet and invasive cervical cancer Verreault R;Chu J;Mandelson M;Shy K https://doi.org/10.1002/ijc.2910430616
  27. Cancer Causes Control v.2 Diet and risk of in situ cervical cancer among white women in the United states Ziegler RG;Jones CJ;Brinton LA https://doi.org/10.1007/BF00052357
  28. J Natl Cancer Inst v.70 Diets and smoking in epidemiology of cancer of the cervix Marshall J;Graham X;Byers T
  29. J Natl Cancer Inst v.80 Nutrients in diet and plasma and risk of in situ cervical cancer Brok K;Berry G;Mock P https://doi.org/10.1093/jnci/80.8.580