Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Aromatic Pollutants by Bacterial Biosensors Bearing Gene Fusions Constructed with the dnaK Promoter of Pseudomonas sp. DJ-12

  • Park, Sang-Ho (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University) ;
  • Lee, Dong-Hun (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University) ;
  • Oh, Kye-Heon (Department of Life Science, Soonchunhyang University) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University)
  • Published : 2002.06.01
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Abstract

Gene fusions were constructed by the transcriptional fusion of the dnaK promoter of pseudomonas sp. DJ-12 or E. coli to the lux or luc marker gene. The dnaKp-DJ::luxCDABE bioluminescent fusion in the biosensor using the Pseudomonas sp. DJ-12 dnaK promoter exhibited about 5-fold more extensive response to ethanol than that of dnaKp-EC::luxCDABE. The bioluminescent response of the dnaK-DJ::luc fusion to ethanol was much weaker than those of the other fusions. The biosensor harboring the dnaKp-DJ::luCDABE fusion was examined for its bioluminescence production based on exposure to aromatic compounds, such as biphenyl, 4-chlorobiphenyl (4CB), 4-hydroxybenzoate (4HBA), and catechol. In particular, the bioluminescence produced by the dnaKp-DJ::luxCDABE fusion was most sensitive to 1 mM biphenyl and 4CB when exposed for 80 min, and the responses were also very strong to other aromatics. Therefore, the biosensor bearing the dnaKp-DJ::luxCDABE fusion would appear to be the most useful for the detection of aromatics and other pollutants.

Keywords

References

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