Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Electron Donors on Nitrate Removal by Nitrate and Nitrite Reductases

  • Yoo, Young-Je (School of Chemical Engineering,) ;
  • Sung, Dong-Won (School of Chemical Engineering) ;
  • Song, Seung-Hoon (Interdisciplinary Program for Biochmical Engineering, and Biotechnology, Seoul National University) ;
  • Kim, Ji-Hyeon (Biotechnology and Chemicals Industries Division, Ministry of Commerce, Industry and Energy, Kyonggi-Do)
  • Published : 2002.03.01
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Abstract

Effects of artificial electron donors to deliver reducing power on enzymic denitrification were investigated using nitrate reductase and nitrite reductase obtained from Ochrobactrum antroyi. The activity of nitrite reductase in the soluble portion was almost the same as that in the precipitated portion of the cell extract. Nitrate removal efficiency was higher with benzyl viologen than with methyl viologen or NADH as an artificial electron donor. The turn-over numbers of nitrate and nitrite reductase were 14.1 and 1.9 umol of nitrogen reduced/min$.$mg cell extracts, respectively when benzyl viologen was used as an electron donor.

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