Effects of Dietary Vitamin E and Selenium Supplementation on Meat Color Stability of Hanwoo(Korean Native Cattle) Bull Beef during Retail Display

Vitamin E와 Selenium의 급여가 비거세우육의 진열저장중 육색 안정성에 미치는 영향

  • 김용선 (강원대학교 동물자원공동연구소) ;
  • 양성운 (강원대학교 축산가공학과) ;
  • 김주용 (강원대학교 축산가공학과) ;
  • 박연수 (강원도 축산기술연구센터) ;
  • 황환섭 (강원도 축산기술연구센터)
  • Published : 2002.06.01

Abstract

The effects of dietary vitamin E and selenium(Se) supplementation on meat color stability in M. Longissimus of Hanwoo(Korean native cattle) bull beef during retail display(5$^{\circ}C$, 1,200 lux) were investigated. Experimental groups were divided into control(Vit E 27 IU/head/day, Se 0.09 mg/head/day), Vit E (2,500 IU/head/day), Se(20 mg/head/day), Vit E+Se(Vit E 2,500 IU/head/day, Se 20 mg/head/day) groups. CIE a*(redness), chroma(C*) values, oxymyoglobin(%) and R630-R580 were significantly (p<0.05) decreased among the 4 treatment groups during retail display, in particular, those values decreased more rapidly in the control group. The metmyoglobin (%) of 0 day(before storage) was not significantly (p<0.05) different among the 4 treatment groups. However, the rate of metmyoglobin accumulation during storage increased more rapidly in the control group. Therefore, discoloration in the control group was more accelerated compared to the other groups. TBARS(thiobarbituric acid reactive substances) which represent lipid rancidity was significantly(p<0.05) lower in Se and Vit E+Se groups than in the control and Vit E groups. Reducing ability of 0 day(before storage) was significantly lower in the control group than in the other groups, and it decreased more rapidly in the control group after 3 days of storage. Consequently, Se-supplemented groups(Se and Vit E+Se groups) were more resistant to lipid oxidation than were the control and Vit E groups. The stability of meat color and myoglobin forms(%) were significantly (p<0.05) higher in Vit E, Se and Vit E+Se groups than in the control group, but there were no difference among Vit E, Se and Vit E+Se groups.

Vitamin E와 selenium의 급여가 거세한우육의 진열저장중 (5$^{\circ}C$, 1,200 lux) 육색안정성에 미치는 영향을 조사하였다. 실험처리구는 대조구(Vit E 27 IU/head/day, Se 0.09 mg/head/day), Vitamin E 급여구(2,500 IU/head/day), Selenium 급여구(20 mg/head/day), Vitamin E와 Selenium 복합 급여구 (Vit E 2,500 IU/head/day, Se 20 mg/head/day)로 나누어 실험하였다. CIE a*값, chroma값, oxymyoglobin함량(%) 및 R630-R580간은 모든 처리구에서 저장기간이 길어질수록 유의적으로 감소하는 경향을 나타내었으며(p<0.05), 특히 대조구가 저장기간이 길어질수록 현저한 감소현상을 보였다. Metmyoglobin(%)의 함량은 저장 전(0일)에는 처리구간에 유의적인 차이를 나타내지 않았으나(p>0.05) 저장기간이 경과 함으로써 대조구의 metmyoglobin 형성율이 현저하게 증가하여 다른 처리구에 비해 변색이 가속화됨을 알 수 있었다. 지질의 산패 정도를 나타내는 TBARS는 Se구와 Vit E+Se구가 대조구와 Vit E구보다 유의적으로 낮은 값을 나타내었다. 환원력은 저장 전에는 대조구가 유의적으로 낮게 나타났으며 3일 저장시 대조구가 다른 처리구에 비해 현저히 낮아짐을 알 수 있었다. 결과적으로 selenium 급여구들(Se구, Vit E+Se 구)의 경우 대조구와 vitamin E급여구에 비해 지질산화에 대해 안정성을 가졌으며, 육색이나 육색소에 있어서는 Vit E구, Se구, Vit E+Se구가 대조구보다 저장안정성을 보였으나 이 3가지 처리구간에는 유의적인 차이가 나타나지 않았다.

Keywords

References

  1. Amold, R. N., Scheller K. K., ArP, S. C., Williams, S. N. and Schaefer, D. M. (1993) Dietary $\alpha$-tocopheryl acetate enhances beef quality in Holstein and beef breed steers. J. Food Sci. 58, 28-33 https://doi.org/10.1111/j.1365-2621.1993.tb03203.x
  2. Buckley, D. J., Morrissey, P. A. and Gray, J. I. (1995) Influence of dietary vitamin E on the oxidative stability and quality of pig meat. J. Anim. Sci. 71, 3122-3130
  3. Chavez, E. R. (1989) Selenium nutrition of pigs; a review. Pig News and Information, 10, 167-171
  4. Demos, B. P., Gerrard, D. E., Mandigo, R. W., Gao, X. and Tan, J. (1996) Mechanically recovered neck bone lean and ascorbic acid improve color stability of ground beef patties. J. Food Sci. 61, 656-659 https://doi.org/10.1111/j.1365-2621.1996.tb13180.x
  5. Faustman, C., Cassens, R. G., Schaefers, D. M., Buege, D. R. and Scheller, K. K. (1989a) Vitamin E supplementation of Holstein steer diets improves sirloin steak color. J. Food. Sci. 54, 485-486 https://doi.org/10.1111/j.1365-2621.1989.tb03114.x
  6. Faustman, C., Cassens, R. G., Schaefers, D. M., Buege, D. R., Williams, S. N. and Scheller, K. K. (1989b) Improvement of pigment and lipid stability in Holstein steer beef by dietary supplementation with vitamin E. J. Food. Sci. 54, 858-862 https://doi.org/10.1111/j.1365-2621.1989.tb07899.x
  7. Govindarajan, S. (1973) Fresh meat color. CRC Crit. Rev. Food Technology, 4, 117-120 https://doi.org/10.1080/10408397309527154
  8. Greene, B. E., Hsin, I. and Zipser, M. W. (1971) Retardation of oxidative color changes in raw ground beef. J. Food Sci. 36, 940-942 https://doi.org/10.1111/j.1365-2621.1971.tb15564.x
  9. Hill, K. E. and Burk, R. F. (1984) Influence of vitamin E and selenium on glutathione-dependent protection against microsomal lipid peroxidation. Biochem. Pharm, 33, 1065-1068 https://doi.org/10.1016/0006-2952(84)90514-8
  10. Kim, Y. Y. (2000) Differences in biological activity and metabolism of selenium due to its chemical form. Kor. J. Anim. Sci. & Technol. 42(6), 835-848
  11. Krzywiki, K. (1979) Assessment ofrelative content of myoglobin, oxymyoglobin and metmyoglobin at the surface of the beef. Meat Sci. 3, 1-5 https://doi.org/10.1016/0309-1740(79)90019-6
  12. Lee, M., Cassens, R. G. and Fennema, O. R. (1981) Effect of metal ions on residual nitrite. J. Food Proc. Preserv. 5, 191-205 https://doi.org/10.1111/j.1745-4549.1981.tb00634.x
  13. Liu, Q., Lanari, M. C., and Schaefer, D. M. (1995) A review of dietary vitamin E supplementation for improvement of beef quality. J. Anim. Sci. 73(10), 3131-3140 https://doi.org/10.2527/1995.73103131x
  14. Mayland, H. F. (1994) Selenium in plant and animal nutrition. In Setenium in the Environment, Frankenberger, W. T. and Benson, S., Marcel Dekker, New York, pp.29-46
  15. Mitsumoto, M.., Ozawa, S., Mitsuhashi, T., Kono, S., Harada, T., Fujita, K. and Koide, K. (1995) Improvement of color and lipid stability during display in Japanese black steer beef by dietary vitamin E supplementation for 4 weeks before slaughter. Anim. Sci. Technol.(Japan) 66, 962-968
  16. Moiseev, I. and Comforth, D. P. (1999) Treatments for prevention of persistent pinking in dark-cutting beef patties. J. Food Sci. 64(4), 738-743 https://doi.org/10.1111/j.1365-2621.1999.tb15122.x
  17. Morrissey, P. A., Buckley, D. J., Sheehy, P. J. A., and Monahan, F. J. (1994) Vitamin E and meat quality. Proc. Nutr. Soc. 53(2), 289-295
  18. National Research Council (2001) Nutrient requirement of beef Cattle. National Academy Press, Washington, D.C.
  19. O'Toole, D. and Raisbeck, M. F. (1995) Pathology of experimentally induced chronic selenosis (alkali disease) in yearling cattle. J. Vet. Diagn. Invest. 7, 364-373 https://doi.org/10.1177/104063879500700312
  20. Renerre, M. (1990) Factors involved with the discoloration of beef. Internat. J. Food Sci. and Technol. 25, 613-630
  21. Renerre, M. and Labas, R. (1987) Biochemical factors influencing metmyoglobin formation in beef muscles. Meat Sci. 19, 151-165 https://doi.org/10.1016/0309-1740(87)90020-9
  22. Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., and Hoekstra, W. G. (1973) Selenium; Biochemical role as a component of glutathione peroxidase. Science, 179, 588-590 https://doi.org/10.1126/science.179.4073.588
  23. SAS (1995) SAS/STAT Software for PC. Release 6.11, SAS Institude, Inc., Cary, NC, U.S.A.
  24. Sinnhuber, R. O. and Yu, T. C. (1977) The 2-thiobarbituric acid reaction, an objective measure of the oxidative deterioration occurring in fats and oils. J. Jap. Soc. Fish. Sci. 26, 259-267
  25. Strange, E. E., Benedicts, R. C., Gugger, R. E., Metzger, V. G. and Swift, C. E. (1974) Simplified methodology for measuring meat color. J. Food Sci. 39, 988-992 https://doi.org/10.1111/j.1365-2621.1974.tb07293.x