Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Clostridium perfringens AB&J and Its Uptake of Bromophenol Blue

  • Kim, Jeong-Dong (The Institute of Life Science and Technology, Sungkyunkwan University) ;
  • An, Hwa-Yong (Department of Food and Life Science, Sungkyunkwan University) ;
  • Yoon, Jung-Hoon (Probionic Co., Korea Research Institute of Bioscience & Biotechnology) ;
  • Park, Yong-Ha (Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Fusako Kawai (Research Institute for Bioresources, Okayama University) ;
  • Jung, Chang-Min (Bioleaders Co., Bio-Venture Center, KRIBB) ;
  • Kang, Kook_-Hee (Department of Food and Life Science, Sungkyunkwan University)
  • Published : 2002.08.01
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Abstract

Several microorganisms from rat and human feces and lumen fluid of cows were screened for their ability to decolorize the synthetic dyes. Consequently, a novel dye-degrading strain AB&J was isolated. Taxonomic identification including 165 rDNA sequencing and phylogenetic analysis indicated that the isolate had 99.9% homology in its 165 rDNA base sequence with Clostridium perfringens. After 27 h Incubation with the strain, brilliant blue R, bromophenol blue, crystal violet, malachite green, methyl green, and methyl orange were decolorized by about 69.3%, 97.7%, 96.3%, 97.9%, 75.1%, and 97.2%, respectively. The triphenlmethane dye, bromophenol blue, was decolorized extensively by growing Clostridium perfringens AB&J cells in liquid cultures under anaerobic condition, although their growth was strongly inhibited in the initial stage of incubation. This group of dyes is toxic, depending on the concentration used. The dye was significantly decolorized at a relatively lower concentration of below 50 $\mu g \;ml^{-1}$, however, the growth of the cells was mostly suppressed at a dye concentration of 100 $\mu g \;ml^{-1}$. The decolorization activity in cell-free extracts was much higher in cytoplasm than in periplasm and cytoplasmic membrane. Therefore, the enzyme related uptake of bromophenol blue seemed to be localized in cytoplasm. The optimal pH and temperature of bromophenol blue uptake fur decolorization activities were 7.0 and 4$0^{\circ}C$, respectively.

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