Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Mannitol Using Leuconostoc mesenteroides NRRL B-1149

  • Kim, Chang-Yong (Department of Material, Chemical and Biochemical Engineering) ;
  • Lee, Jin-Ha (Faculty of Applied chemical Engineering) ;
  • Kim, Byung-Hoon (Engineering Research Institute) ;
  • Yoo, Sun-Kyun (Department of Food and Biotechnology, Joong Bu University) ;
  • Seo, Eun-Seong (Department of Material, Chemical and Biochemical Engineering) ;
  • Cho, Kab-Su (Department of Molecular Biotechnology, Chonnam National University) ;
  • Donal F. Day (Audubon Sugar Institute, Louisiana State University) ;
  • Kim, Doman (Department of Material, Chemical and Biochemical Engineering, Faculty of Applied Chemical Engineering)
  • Published : 2002.07.01
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Abstract

A process for the production of mannitol from fructose (5% to 25%) using Leuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out In batch or fed-batch fermentations without aeration at 28$\^{C}$, pH 5.0. When 5% fructose was used In batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the frurtose concentration was Increased to 10%, the yield dropped to 59.6% of the theoretical value. However, In the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fruttose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply the final yield was 83.7% of the theoretical yield. When 20% fructose was used In the same manner, the yield was 89.5% of theoretical yield.

Keywords

References

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