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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of $\beta$-1,3/1,6-glucan by Aureobasidium pullulans SM-2001

  • 서형필 (동아대학교 생명자원과학대학 생물공학전공) ;
  • 김지모 (동아대학교 생명자원과학대학 생물공학전공) ;
  • 신현동 (경북대학교 자연과학대학 유전공학과) ;
  • 김태권 (경북대학교 자연과학대학 유전공학과) ;
  • 장희정 (㈜글루칸) ;
  • 박복련 (㈜글루칸) ;
  • 이진우 (동아대학교 생명자원과학대학 생물공학전공)
  • Published : 2002.08.01
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Abstract

Production of the exopolymer by Aureobasidium pullulans SM-2001, UV induced mutant of A. pullulans ATCC 42023, was investigated. The exopolymer produced by A. pullulans SM-2001 was confirmed to be ${\beta}$-1,3-linked homoglucans containing a few ${\beta}$-1,6-linked single glucosyl branches(${\beta}$-1,3/1,6-glucan) with the nuclear magnetic resonance(NMR) spectrum. The average molecular weight of ${\beta}$-1,3/1,6-glucan produced by A. pullulans SM-2001 was about 2.6 ${\times}$ 10$\^$5/ by the gel permeation chromatographic analysis. Sucrose was known to be better carbon source for the production of ${\beta}$ -1,3/1,6-glucan than other tested carbon sources in this study. Maximal conversion rate of ${\beta}$-1,3/1,6-glucan was about 50% when the carbon source was 0.5%(w/v) sucrose.

항암, 항콜레스테롤, 항산화 및 면역증강 효과와 피부재생 효과 등 여러 가지 생리활성이 밝혀지고 있는 $\beta$-1,3/1,6-글루칸은 크게 식물성 유래, 효모 및 곰팡이 유래, 버섯 유래의 것으로 등으로 분류할 수 있다. 풀루란을 생산하는 Aureobasidum pullulans ATCC 42023에 자외선을 조사하여 얻은 변이주인 Aureobasidum puiluian SM-2001 균주가 생산하여 체외로 분비하는 고분자 중합체를 핵자기 공명분석기로 분석한 결과, $\beta$-1,3- 및 $\beta$-1,6- 결합이 서로 혼재되어 있는 $\beta$-1,3/1,6-글루칸의 전형적인 구조임을 확인하였으며, 평균 분자량은 2.6$\times$$10^{5}$ 임을 확인하였다. 또한, $\beta$-1,3/1,6-글루칸의 생산에 최적인 탄소원이 설탕임을 확인하였으며, 0.5% (w/v)의 설탕을 탄소원으로 사용하였을 경우 약 50%의 변환율로 $\beta$-1,3/l,6-글루칸을 생산할 수 있었다. 이는 생물공학적인 방법으로 $\beta$-1,3/l,6-글루칸의 생산을 의미하며 저렴한 생산비로 대량 생산할 수 있는 방법의 개발을 의미한다한다

Keywords

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