Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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groES Expression Related to Antifungal Activity of Streptomyces sp. SAR01

Streptomyces sp. SAR01 균주에서의 항진균 관련 groES의 발현

  • 이영근 (한국원자력연구소 R. 방사선응용연구팀) ;
  • 김재성 (한국원자력연구소 R. 방사선응용연구팀) ;
  • 조규성 (한국원자력연구소 R. 방사선응용연구팀) ;
  • 장병일 (한국원자력연구소 R. 방사선응용연구팀) ;
  • 추철형 (한국원자력연구소 R. 방사선응용연구팀)
  • Published : 2002.09.01
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Abstract

To analyse proteins and gene related to antifungal activity, SAR01 strain was isolated from a brown seaweed and identified as Streptomyces sp. by FAME(fatty acid methyl ester) analysis. Antifungal activity deficient mutant(SAR535) of Streptomyces sp. SAR01 was induced by gamma radiation$({60}^Co)$. It was found that 6 specific protein spots appeared only in SAR01 by 2-D electrophoresis analysis. Among them, a protein of 10 kDa had homology of 96% with 10 kD chaperonin cpn 10 (GroES) by Basic Local Alignment Search Tool(BLAST, NCBI) analysis. SAR535 transformants into which groES was transferred by electroporation revealed antifungal activity newly similar with SAR01 It suggested that groES be supposed to be related to the antifungal activity of Streptomyces sp. SAR01.

항진균 활성 관련 단백질을 찾기 위해 미역류로부터 Streptomyces sp. SAR01을 분리하고, FAME (fatty acid methyl ester)분석으로 동정하였다. 감마선(sup $({60}^Co)$)조사를 이용하여 항진균 활성 결핍 돌연변이체(SAR535)를 유도한 후, 이차원전기영동으로 단백질을 분석한 결과 SAR01에만 존재하는 6종의 단백질을 확인할 수 있었다. 이중 10 kDa chaperonin cpn10 (GroES)과 96%의 상동성을 가진 10 kDa의 단백질을 클로닝하였으며, E. coli Ml5에서 과대 발현됨을 확인하였다. 또한 SAR535에 형질전환시킨 결과 SAR01과 유사한 항진균 활성이 나타났다. 이것으로 볼 때 groES는 Streptomyces sp. SAR01의 항진균 활성에 관련된 것으로 사료된다.

Keywords

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