Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Probiotic Properties of Enterococcus durans LP44 Isolated from Pigs Feces

돼지의 분변에서 분리한 Enterococcus durans LP44의 생균제로서의 특성

  • 홍종욱 (단국대학교 동물자원과학과) ;
  • 김인호 (단국대학교 동물자원과학과) ;
  • 이상환 (단국대학교 동물자원과학과) ;
  • 권오석 (단국대학교 동물자원과학과) ;
  • 한영근 (농협중앙회) ;
  • 김지훈 (애그리브랜드푸리나코리아) ;
  • 강국희 (성균관대학교 식품생명자원학과)
  • Published : 2002.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

We have screened the microorganisms from pigs feces for the development of probiotics which have acid-and bile-tolerance. Among them, a strain which was identified as Enterococcu durans LP44 was selected. Sixty Duroc$\times$Yorkshire$\times$Landrace pigs (6.30$\pm$0.04 kg average initial body weight) were used in a 14 days growth assay to determine the effects of Enterococco duran Lf44 culture on growth performance of weaned pigs. Dietary treatments included 1) DF (dry feeding),2) W (wet feeding),3) WF+5ED (wet feeding+5% Enterocucu duran LP44 culture),4) WF+ 10ED (wet feeding +10% Enterococcus durans LP44 culture). For overall periods, average daily gain in pigs fed % diet tended to increase compared to pigs fed DF diet without significant difference. Also, there was no significant difference in gain/feed. At 5 days after the onset of the experiment, pigs fed WF+5ED and WF+10ED diets were significantly increased in dry matter and nitrogen digestibilities compared to pigs fed DF and W diets (p<0.05). At 14 days after the onset of the experiment, pigs fed WF+ 10ED diet were significantly increased in dry matter digestibility compared to pigs fed DF diet (p<0.05). Also, pigs fed WF + 10ED diet were significantly increased in nitrogen digestibility compared to other treatments (p<0.05). In conclusion, supplemental Enterococcus durans LP44 was an effective means of improving ADG and nutrient digestibility.

본 시험은 국내 사육 돼지 분변에서 분리, 동정한 E. duran LP44의 생균제로서의 효과 및 습식급이 형태로 생균제를 급여하였을 경우, 생균제 급여 이후 자돈의 성장에 미치는 영향을 조사하기 위하여 실시하였다. 돈분으로부터 180균주의 혐기성 세균을 분리한 후, 내산성 및 내담즙성이 있는 E. duran LP44균주를 선발하였다 E. durans LP44 배양물을 이유자돈에게 급여한 사양시험에서, 전체사양 시험기간 동안, 일당증체량 및 일당식이 섭취량에 있어서는 WF 처리군이 DF 처리구와 비교하여 높게 평가되었으나, 통계적으로 유의적인 차이를 보이지 않았다. 또한, 식이효율에 있어서도 처리구간에 유의적인 차이를 보이지 않았다. 또한, 영양소 소화율에 있어서는 사양시험 개시 후 5일째 되는 날에 건물과 질소 소화율은 WF+SED군과 WF+10ED군이 DF군과 WF군과 비교하여 통계적으로 높게 평가되었다(p<0.05). 또한, 14일째 되는 날, 건물 소화율에 있어서는 WF+10ED군이 DF군과 비교하여 유의적으로 높게 평가되었으며, 질소 소화율에 있어서는 WF+10ED군이 다른 처리군과 비교하여 통계적으로 높게 평가되었다(p<0.05). 결론적으로 국내 사육 돼지 분변에서 분리, 동정한 E. duran LP44 배양물을 습식급이 형태로 급여한 후 자돈 성장에 미치는 영향에 대한 시험에서,E. durans LP44 배양물을 급여하였을 경우 자돈 시기에 성장 및 영양소 소화율을 향상시키는 것으로 사료된다.

Keywords

References

  1. Chang YH, Kim JK, Kim HJ, Kim WY, Kim YB, Park YH. 2000. Probiotic effects of Lactobacillus reuteri BSA-131 on piglets. Kor J Appl Microbiol Biotechnol 28: 8-13.
  2. Fuller R. 1989. Probiotics in man and animals A review. J Appl Bacteriol 66: 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  3. Xuan ZN, Kim JD, Heo KN, Jung HJ, Lee JH, Han YK, Kim YY, Han IK. 2001. Study on the development of a probiotics complex for weaned pigs. Asian-Aust J Anim Sci 14: 1425-1428. https://doi.org/10.5713/ajas.2001.1425
  4. Hill IR, Kenworthy R, Porter P. 1970. Studies of the effect of dietary lactobacilli on intestinal and urinary amines in pigs in relation to weaning and postweaning diarrhea. Res Vet Sci 11: 320-326.
  5. Shahani KM, Valki JR, Kilara A. 1976. Natural antibiotic activity of Lactobacillus acidophilus and bulgaricus: I. Cultured conditions for the production of antibiotic. J Cultured Dairy Prod 11: 14-19.
  6. Muralidhara KS, Sheggeby GG, Eliker PR, England DC, Sandine WE. 1977. Effects of feeding lactobacilli on the coliform and Lactobacillus flora on intestinal tissue and feces from piglets. J Food Prod 40: 288-291. https://doi.org/10.4315/0362-028X-40.5.288
  7. Noh SH, Moon HK, Kan IK, Shin IS. 1995. Effect of dietary growth promoting substances on the growth performance in pigs. Kor J Anim Sci 37:66-72.
  8. Kim JH, Kim CH, Ko YD. 2001. Effect of dietary supplementation of fermented feed (Bio-) on performance of finishing pigs and fecal ammonia gas emission. J Anim Sci Technol Kor 43: 193-202.
  9. AOAC. 1995. Official method of analysis. 16th ed. Association of official analytical chemists, Washington, DC.
  10. SAS. 1996. SAS user's guide. Release 6.12 ed. SAS institute, Inc., Cary, NC.
  11. Duncan DB. 1955. Multiple range and multiple F tests. Biometrics 11: 1-42.
  12. Gonyou HW, Lou Z. 2000. Effects of eating space and availability of water in feeders on productivity and eating behavior of grower/finisher pigs. J Anim Sci 78: 865-870. https://doi.org/10.2527/2000.784865x
  13. Veum TL, Bowman GL. 1973. Saccharomyces cerevisiae yeast culture in diets for mechanically-fed neonatal piglets and early growing self-fed pigs. J Anim Sci 37: 67-71. https://doi.org/10.2527/jas1973.37167x
  14. Kornegay ET, Rhein-Welker D, Lindemann MD, Wood CM. 1995. Performance and nutrient digestibility in weanling pigs as influenced by yeast culture additions to starter diets containing dried whey or one of two fiber sources. J Anim Sci 73: 1381-1389. https://doi.org/10.2527/1995.7351381x
  15. Underdahl NR, Torres-Median A, Doster AR. 1982. Effect of Streptococcus faecium C-68 in the control of Escherichia coli-induced diarrhea in gnotobiotic pigs. J Vet Res 43: 2227-2232.