Journal of Asia-Pacific Entomology

Korean Society of Applied Entomology (한국응용곤충학회)
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A Pathogenic Bacterium, Enterococcus faecalis, to the Beet Armyworm, Spodoptera exigua

  • Park, Youngjin (School of Bioresource Sciences, Andong national University) ;
  • Kim, Kilho (Department of Sericulture and Entomology Resources, Sangju national University) ;
  • Kim, Yonggyun (School of Bioresource Sciences, Andong national University)
  • Published : 2002.11.01
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Abstract

A bacterial disease was found in the beet armyworm, Spodoptera exigua (Hubner). Blackened body of the infected larvae was a typical symptom of the epizootic disease especially at the intersegmental areas. We isolated the bacteria from the hemolymph of the infected 5th instar larvae and identified the isolate as a gram-positive bacterium, Enterococcus faecalis. When the 4th instar larvae were injected with the bacteria, half lethal dose of the bacteria was estimated as 22,593 colony-forming units (cfu) per larva and half lethal time of the bacteria was estimated as 2 days at $1O^7$ cfu injection and 6 days at $1O^8$ cfu injection. The bacteria were strongly resistant to each 1,000 ppm of ampicillin, kanamycin, and streptomycin. They were, however, relatively susceptible to mixture (1,000 ppm) of different combinations of the three antibiotics.

Keywords

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