Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Chitosan-Soybean Curd on Serum Lipid Metabolism in Rats Fed High-Fat Diet

키토산 두부가 고지질 식이를 급여한 흰쥐의 혈청 지질대사에 미치는 영향

  • 노홍균 (대구카톨릭대학교 식품공학과) ;
  • 백경연 (대구카톨릭대학교 식품공학과) ;
  • 김석중 (대구카톨릭대학교 식품공학과)
  • Published : 2002.12.01
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Abstract

Effects of chitosan-soybean curd (prepared using chitosan as a coagulant), commercial soybean curd and chitosan powder on serum lipid metabolism were investigated with rats fed high-fat diet for 6 weeks. Food intake of experimental groups fed high-fat (HF), chitosan-soybean curd (CSC), soybean curd (SC), or chitosan powder (CP)diet was lower compared to t]tat of normal group. Body weight gain was lower in rats fed the CSC, SC and CP diets than in rats fed the HF diet. There was no significant difference in total cholesterol concentration among all groups, but HDL-cholesterol concentration was higher and LDL-cholesterol concentration was lower in rats fed the CSC, SC, CP diets than in rats fed the HF diet. Total lipid level was lower in rats fed the SC and CP diets than in rats fed the HF diet. Increment of triglyceride level and decrement of phospholipid level in rats fed the HF diet were recovered significantly by the CSC, SC and Cf diet. Our results indicate that chitosan-soybean curd prepared under the commercial processing condition may provide the beneficial effect on lipid metabolism even though its effect didn't show any significant difference compared to that of commercial soybean curd under the present experimental conditions.

키토산을 응고제로 사용하여 상업 적 조건에서 제조한 두부와 일반 두부 및 키토산 분말을 고지질 급여 흰쥐 에6주간 급여한 후 혈청 지질대사에 미치는 영향을 조사하였다. 그 결과, 흰쥐의 식이 섭취량은 정상 식이군(N)에 비해 모두낮았으며, 키토산 두부 첨가군(CSC)과 키토산 분말 첨가군(CP)의 식이섭취량이 특히 낮았다. 체중 증가량은 고지질 식이군(HF)에 비해 CSC군, 일반 두부 첨가군(SC) 그리고 CP군들은 모두 유의적으로 낮았으며, 특히 CP군이 가장 낮은 체중증가를 나타내었다. 혈청중의 총 콜레스테롤 농도는 전 실험군간에 유의적인 차이는 없었으나, HDL-콜레스테롤 함량에서는 CSC, SC, CP군 모두 HF군에 비하여 높게 나타났다 따라서 총 콜레스테롤중 HDL-콜레스테롤이 차지하는 비율은 CSC, SC, CP군이 HF군에 비하여 높고 동맥경화지수는 반대로 낮게 나타났다. LDL-콜레스테롤 농도는 HF군에 비해 CSC, SC, CP 실험 군에서 유의적으로 낮게 나타났다. 총 지질 함량에서는 HF군에 비하여 SC와 CP군에서 유의적으로 감소하였다. 고지질 식이에 의해 증가한 중성지질 및 감소한 인지질 함량은 CSC, SC, CP군에서 유의적으로 회복되었다. 본 실험 에서는 HF군에 비해 CP군 뿐 아니라 CSC, SC군에서 HDL-콜레스테롤 및 인지질 농도의 증가, 중성지질 및 LDL-콜레스테롤 농도의 감소가 관찰됨으로써 상업용 키토산 두부가 고지질 관련 성인병 예방 및 치료에 효과가 있을 것으로 사료되었다. 그러나 현 실험조건하에서는 일반두부와 키토산 두부간에 효능에서 유의적인 차이를 발견하긴 어려웠다.

Keywords

References

  1. Dotson CR, Frank HA, Cavaletto CG. 1977. Indirect methods as criteria of spoilage in tofu (soybean curd). J Food Sci 42: 273-274. https://doi.org/10.1111/j.1365-2621.1977.tb01270.x
  2. Kovats SK, Doyle MP, Tanaka N. 1984. Evaluation of the microbiological safety of tofu. J Food Prot 47: 618-622. https://doi.org/10.4315/0362-028X-47.8.618
  3. Lee KS, Kim DH, Baek SH, Choun SH. 1990. Effects of coagulants and soaking solutions of tofu (soybean curd) on extending its shelf-life. Korean J Food Sci Technol 22: 116-122.
  4. Kim DH, Lee KS. 1992. Effects of coagulants on storage of packed tofu. Korean J Food Sci Technol 24: 92-96.
  5. Wu MT, Salunkhe DK. 1977. Extending shelf-life of fresh soybean curds by in-package microwave treatments. J Food Sci 42: 1448-1450. https://doi.org/10.1111/j.1365-2621.1977.tb08396.x
  6. Pontecorvo AJ, Bourne MC. 1978. Simple methods for extending the shelf-life of soy curd (tofu) in tropical areas. J Food Sci 43: 969-972. https://doi.org/10.1111/j.1365-2621.1978.tb02464.x
  7. Miskovsky A, Stone MB. 1987. Effects of chemical preservatives on storage and nutrient composition of soybean curd. J Food Sci 52: 1535-1537. https://doi.org/10.1111/j.1365-2621.1987.tb05873.x
  8. No HK, Meyers, SP. 2001. Use of chitosan as a coagulant in tofu preparation. Poster (44E-22) presentation at the 2001 IFT Annual Meeting, New Orleans, USA.
  9. Lee KS, No HK, Meyers SP. 2001. Effect of chitosan as a coagulant on shelf-life of tofu prepared in commercial-scale. Food Sci Biotechnol 10: 529-533.
  10. Muzzarelli RAA. 1977. Chitin. Pergamon, Oxford.
  11. Knorr D. 1984. Use of chitinous polymers in food-a challenge for food research and development. Food Technol 38: 85-97.
  12. Sugano M, Fujikawa T, Hiratsuji Y, Nakashima K, Fukuda N, Hasegawa Y. 1980. A novel use of chitosan as a hypocholesterolemic agent in rats. Am J Clin Nutr 33: 787-793. https://doi.org/10.1093/ajcn/33.4.787
  13. Sugano M, Yoshida K, Hashimoto M, Enomoto K, Hirano S. 1992. Hypocholesterolemic activity of partially hydrolyzed chitosan in rats. In Advances in chitin and chitosan. Brine CJ, Sandford PA, Zikakis JP, eds. Elsevier, London. p 472-478.
  14. No HK, Park NY, Lee SH, Meyers SP. 2002. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 74: 65-72. https://doi.org/10.1016/S0168-1605(01)00717-6
  15. Sudarshan NR, Hoover DG, Knorr D. 1992. Antibacterial action of chitosan. Food Biotechnol 6: 257-272. https://doi.org/10.1080/08905439209549838
  16. Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S, Suzuki M. 1988. Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor. Chem Pharm Bull 36: 784-790. https://doi.org/10.1248/cpb.36.784
  17. Youn SK, Kim YJ, Ahn DH. 2001. Antioxidative effects of chitosan in meat sausage. J Korean Soc Food Sci Nutr 30: 477-481.
  18. Sugano M, Watanabe S, Kishi A, Izume M, Ohtakara A. 1988. Hypocholesterolemic action of chitosans with different viscosity in rats. Lipids 23: 187-191. https://doi.org/10.1007/BF02535456
  19. Ebihara K, Schneeman BO. 1989. Interaction of bile acids, phospholipids, cholesterol and triglyceride with dietary fibers in the small intestine of rats. J Nutr 119: 1100-1106. https://doi.org/10.1093/jn/119.8.1100
  20. Vahouny GV, Satchithanandam S, Cassidy MM, Lightfoot FB, Furda I. 1983. Comparative effects of chitosan and cholestyramine on lymphatic absorption of lipids in the rat. Am J Clin Nutr 38: 278-284. https://doi.org/10.1093/ajcn/38.2.278
  21. Ikeda I, Sugano M, Yoshida K, Sasaki E, Iwamoto Y, Hatano K. 1993. Effects of chitosan hydrolysates on lipid absorption and on serum and liver lipid concentration in rats. J Agric Food Chem 41: 431-435. https://doi.org/10.1021/jf00027a016
  22. National Cholesterol Education Program. 1988. Report of the national cholesterol education and treatment of high blood cholesterol in adult. Arch Int Med 148: 36-39. https://doi.org/10.1001/archinte.148.1.36
  23. Lee JM, Cho WK, Park HJ. 1998. Effects of chitosan treated with enzymatic methods on glucose and lipid metabolism in rats. Korean J Nutr 31: 1112-1120.
  24. Park JR, Moon IS, Choi SH, Shon MY. 1999. Effects of chitin and chitosan on lipid metabolism in rats. J Korean Soc Food Sci Nutr 28: 477-483.
  25. Chung GH, Kim BS, Hur JW, Chung SY. 1996. Effect of dietary lobster shrimp chitosan on lipid metabolism in dietinduced hyperlipidemic rats. J Korean Soc Food Nutr 25: 384-391.
  26. Aoyama, Y, Matsumoto H, Tsuda T, Ohmura E, Yoshida A. 1988. Effect on liver and serum lipids in rats of dietary additions of fibers and cholestyramine to a cystine-excess diet. Agric Biol Chem 52: 2811-2816. https://doi.org/10.1271/bbb1961.52.2811
  27. Fukada Y, Kimura K, Ayaki Y. 1991. Effect of chitosan feeding on intestinal bile acid metabolism in rats. Lipids 26: 395-399. https://doi.org/10.1007/BF02537206
  28. Gordon DT, Besch-Williford C. 1984. Action of amino polymers on iron status, gut morphology, and cholesterol levels in the rat. In Chitin, chitosan, and related enzymes. Zikakis JP, ed. Academic Press, Orlando. p 97-117.
  29. Lee JM, Son BK. 1998. Effects of chitosan of different molecular weights on lipid metabolism in rats. Korean J Nutr 31: 143-152.
  30. Choi YS, Lee SY. 1993. Cholesterol-lowering effects of soybean products (curd or curd residue) in rats. J Korean Soc Food Nutr 22: 673-677.
  31. Kim CH, Park JS, Sohn HS, Chung CW. 2002. Determination of isoflavone, total saponin, dietary fiber, soy oligosaccharides and lecithins from commercial soy products based on the one serving size-some bioactive compounds from commercialized soy products. Korean J Food Sci Technol 34: 96-102.
  32. Lee YJ, Park MH, Hwang SW, Bae MJ, Han JP. 1994. Effect of pine pollen on serum and liver lipids in rats on a fed high fat diet. J Korean Soc Food Sci Nutr 23: 192-197.
  33. Park HY, Yoon HD, Oh EK. 2001. Effect of Meristotheca papulosa on lipid concentration of serum and liver in rats fed high fat diet. J Korean Soc Food Sci Nutr 30: 107-111.
  34. Han EK, Sung IS, Moon HG, Cho SY. 1998. Effect of Cordonopsis lanceolata water extract on the levels of lipid in rats fed high fat diet. J Korean Soc Food Sci Nutr 27: 940-944.
  35. Kim JY, Kim WK, Kim HY, Kim MH, Kim SH. 2000. Effects of soy oligosaccharides and varing dietary fats on intestinal microflora, lipid profiles and immune responses in rats. Korean J Nutr 33: 597-612
  36. Narayan KA, McMullen JJ. 1979. The interactive effect of dietary glycerol and corn oil on rat liver lipids, serum lipids and serum lipoproteins. J Nutr 109: 1836-1846. https://doi.org/10.1093/jn/109.11.1836
  37. Narayan KA, McMullen JJ, Wakefield T, Calhoun WK. 1977. Influence of dietary glycerol on the serum lipoproteins of rats fed a fat-free diet. J Nutr 107: 2153－2163. https://doi.org/10.1093/jn/107.12.2153
  38. Oda T, Shikata T, Naito C, Suzuki H, Kanetaka T. 1970. Phospholipid fatty liver: a report of three cases with a new type of fatty liver. Jpn J Exp Med 40: 127-140.
  39. Kim MK, Seol EY. 1994. Effect of dietary chitin and chitosan on cadmium toxicity and lipid metabolism in rats. Korean J Nutr 27: 996-1006.

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