Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Detection of Mycobacterium bovis in cattle by PCR

PCR기법을 이용한 젖소 결핵균 검색 분리 조사

  • 공신국 (충청남도축산위생연구소 통합지소) ;
  • 이건택 (충청남도축산위생연구소 통합지소) ;
  • 임종묵 (충청남도축산위생연구소 통합지소) ;
  • 양승민 (충청남도축산위생연구소 통합지소) ;
  • 이요안나 (충청남도축산위생연구소 통합지소) ;
  • 문순화 (충청남도축산위생연구소 통합지소)
  • Published : 2002.06.01
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Abstract

The purpose of this study was to detect Mycobacterium bovis in cattle(serum, milk, lung, lymph node) by PCR. Nineteen samples from 7 skin test-positive cattle were analyzed. The amplified band of IS6110 by PCR was detected from 2 samples in lung and Iymph node. But the sensitivities of the present methods for detecting M bovis in milk and serum are deficient. Because the PCR sensitivity has been shown to be hindered by the method used to isolate the nucleic acid target. PCR-based methods have the potential to be faster, more accurate, and the most efficient means of detecting M bovis. The detection of M bovis by PCR will contribute to the more efficient detection and control of tuberculosis.

Keywords

References

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