Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effects of Low Dose $\gamma$-Radiation on the Growth, Activities of Enzymes and Photosynthetic Activities of Gourd (Lagenaria siceraria)

저선량 $\gamma$선 조사가 참박의 초기 생육과 효소 활성 및 광합성 능에 미치는 영향

  • 이혜연 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 김재성 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 백명화 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 이영근 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 임돈순 (한국원자력연구소 동위원소 방사선응용연구팀)
  • Published : 2002.09.01
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Abstract

Gourd seeds were irradiated with the doses of 0-20 Gy to investigate the effect of the low dose $\gamma$-radiation on the early growth and physiological activity. The stimulating effects of the low dose y- radiation on the early growth were not noticeably high, but were increased generally at 4-16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with $\gamma$- radiation were increased at 8 Gy irradiation group. The photochemical activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSII, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4 hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Em in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose $\gamma$- radiation. Changes in the effective quantum yield of PSII, $\varphi_{PSII}$ and 1/Fo- l/Fm, a measure of the rate constant of excitation trapping by the PSII reaction center, showed similar pattern to Fv/Em. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose $\gamma$- radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.

저선량 $\gamma선 조사가 참박의 초기생육과 생리활성에 미치는 효과를 알아보기 위하여 참박 종자에 $\gamma선을 0-2O Gy 수준으로 조사하여 생육을 관찰하였다. 저선량 $\gamma선에 의한 참박의 초기생육은 대조구에 비해 4-16Gy에서 다소 증가하였고, 생육 조사시 측정한 자엽의 경우 catalase와 Peroxidase 활성이 대조구에 비해 8 Gy 조사구에서 가장 높았으며 본엽은 peroxidase활성이 4 Gy 조사구에서 확연히 증가하였다. 저선량 $\gamma선을 조사한 박 식물체의 광 스트레스에 대한 반응효과는 광계II의 광화학적 효율이 대조구와 8 Gy의 경우 50% 정도 감소되었으나 4 Gy에서는 40% 정도의 감소를 보였다. 반면 Fo는 광저해가 진행됨에 따라 약간의 증가를 보였으나 대조구나 저선량 조사구사이의 차이는 거의 없는 것으로 나타났다. 광계II의 광양자 수율, $\varphi_{PSII}$과 광계II 반응중심의 흥분 포획능, 1/Fo-l/Fm 또한 광저해가 진행되는 동안 감소하여 $\varphi_{PSII}$는 대조구와 8 Gy의 경우 20% 정도 감소를 보인 반면 4 Gy 조사구는 15%의 감소를 보였고, 1/Fo-lF/m은 대조구와 8 Gy는 55%, 4 Gy는 45%의 감소를 나타냈다. 비광화학적 소멸인 NPQ는 대조구와 저선량 조사구 모두 70% 정도 감소되는 경향을 보였다. 이러한 결과를 볼 때 종자의 종피를 투과한 저선량의 $\gamma선이 참박의 생육을 촉진시키고 4 Gy에서 광 스트레스에 대한 저해가 감소되었다.

Keywords

References

  1. 한국환경농학회지 v.16 전리방사선에 의한 배추와 무의 생육촉진 효과 김재성;김진규;이은경;이영복
  2. 한국환경생물학회지 v.17 묵은 배추종자의 발아와 생육에 미치는 저선량 γ선 효과 김재성;이영근;백명화;이영복;박영선
  3. 한국환경생물학회지 v.17 저선량 감마선이 땅콩 생장에 미치는 효과 김재성;이은경;백명화;박홍숙;김광호
  4. 한국환경농학회지 v.19 저선량 방사선이 참박의 초기생육과 생리활성에 미치는 효과 김재성;이영근;박홍숙;백명화;정규희
  5. 한국환경농학회지 v.19 옥수수 생육에 미치는 저선량 감마선 조사효과 김재성;이영근;박홍숙;백명화;김동희
  6. 한국원예학회지 v.39 저선량 감마선 조사에 의한 고추의 발아와 생육 이은경;김재성;이영근;이영복
  7. In Ecology of photosynthesis in Sun and Shade Thylakoid membrane organisation in Sun/Shade Acclimation Anderson JM;MS Chow;DJ Goodchild;Evans JR (ed.);SV Caemmerer (ed.);WW Adams III (ed.)
  8. Plant Cell Environ. v.9 Relationship between chloroplast structure and O$_2$ evolution rate of leaf discs in plants from different biotypes in south Finland Aro EM;E Rintamaki;P Korhonen;P Maenpaa https://doi.org/10.1111/j.1365-3040.1986.tb01570.x
  9. Anal Biochem v.72 A rapid sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford MM https://doi.org/10.1016/0003-2697(76)90527-3
  10. Biochim. Biophys. Acta. v.548 A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts Briantais JM;C Vernotte;M Picaud;GH Krause https://doi.org/10.1016/0005-2728(79)90193-2
  11. Curr. Opi. Biotech. v.6 Oxidative stress in plants Dirk I;MV Montagu https://doi.org/10.1016/0958-1669(95)80024-7
  12. Plant Cell Environ. v.17 Protection against oxygen radicals: an important defense mechanism studied in transgenic plants Foyer CH;P Descourvieres;KJ Kunert https://doi.org/10.1111/j.1365-3040.1994.tb00146.x
  13. Indian J. Agric. Sci. v.42 Effect of presowing gamma irradiated seeds in relation to the germination behavior of Indian colza (Brassica campestris L. var. Sarson Prain) Garg CK;B Tirwari;O Singh
  14. Photochem. Photobiol. v.40 mechanism of photoinactivation of plant plasma membrane ATPase Imbrie CW;TM Murphy https://doi.org/10.1111/j.1751-1097.1984.tb04582.x
  15. Photosynthetica v.15 Photosynthetic activity and distribution of photoassimilated $^{14}$C in seedlings of Zeamays grown from gamma-irradiated seeds Koepp R;M Kramer
  16. Ann. rev. Plant Physiol. Plant Mol. Biol. v.42 Chlorophyll fluorescence and photosynthesis: The basis Krause GH;E Weis https://doi.org/10.1146/annurev.pp.42.060191.001525
  17. Physiol. Plant. v.105 Photoinactivation of photosystem II in leaves of Capsicum annuum Lee HY;WS Chow;YN Hong
  18. Hormesis with ionizing radiation Luckey TD
  19. Manual on Radiation Dosimetry Niels WH;JB Roger
  20. Biochim. Biophys. Acta. v.975 Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyll a and b with four different solvents: verification of the concentration of chlorophy by atomic absorption spectroscopy Porra RJ;WA Thompson;PE Kriedemann https://doi.org/10.1016/S0005-2728(89)80347-0
  21. Plant. Physiol. v.110 Ultraviolet-B and ozone induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana Rao MV;G Paliyath;DP Ormrod https://doi.org/10.1104/pp.110.1.125
  22. In Light Emission by Plants and Bacteria Practical applications of fluorometric methods to algae and higher plant research Renger G;U Schreiber;Govindjee D (ed.);J. Amesz (ed.);DC Fork (ed.)
  23. Int. J. Radiat. Bio. v.69 Peroxidase changes in barley induced by ionizing and thermal radiation Sah NK;S Pramanik;SS Raychaudhuri https://doi.org/10.1080/095530096146237
  24. Photosynth. Res. v.10 Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer Schreiber U;U Schliwa;W Bilger https://doi.org/10.1007/BF00024185
  25. Inst. Fiziol. Biokhim. Rast. Effect of ionizing radiation on the physiological-biochemical properties and metabolism of agricultural plants Vlasyuk PA
  26. Phytochemistry v.48 Antioxidant enzyme in Nicotiana cells containing an Ipomoea peroxidase gene Yun BW;HG Hue;SY Kwon;HS Lee;JK Jo;SS Kwak https://doi.org/10.1016/S0031-9422(98)00010-7
  27. Vestsel'Skokhoz Nauk v.8 Studies on the mechanism of the biological effect of small doses of ionizing radiation in plant Zhezhel NG