KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Antimicrobial Effects of Herbal Medicine Extracts on Staphylococcus aureus and Escherichia coli O157:H7

생약재 추출물의 Staphylococcus aureus 와 Escherichia coli O157:H7에 대한 항균효과

  • Cai, Hua (Department of Biological Engineering and Institute of Industrial Biotechnology, Inha University) ;
  • Choi, Soo-Im (Department of Biological Engineering and Institute of Industrial Biotechnology, Inha University) ;
  • Lee, Yun-Mi (Department of Biological Engineering and Institute of Industrial Biotechnology, Inha University) ;
  • Heo, Tae-Ryeon (Department of Biological Engineering and Institute of Industrial Biotechnology, Inha University)
  • 채화 (인하대학교 생물공학과) ;
  • 최수임 (인하대학교 생물공학과) ;
  • 이윤미 (인하대학교 생물공학과) ;
  • 허태련 (인하대학교 생물공학과)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

To screening of antimicrobial activity, 95% ethanol and hot water extracts of roots, fruits, leaves, radix and stems of 50 species of traditional herbal medicines were examined. For their growth inhibitory effects on two food-born microorganisms, S. aureus and E. coli O157:H7, by the paper disc diffusion method and the minimum inhibitory concentration(MIC) test. Moutan radicis Cortex and Achyranthis Radix showed the highest inhibitory activities on both S. aureus and E. coli O157:H7. The Inhibition zones of Moutan radicis Cortex on S. aureus and E. coii O157:H7 were 22 mm and 24 mm respectively, and the corresponding inhibition zone of Achyranthis Radix were 23 mm and 22 mm. The MIC or Achyranthis Radix on S. aureus was 156.25 $\mu$g/mL, and the MIC or Achyranthis Radix and Moutan radicis Cortexas on E. coli O157:H7 were 625 $\mu$g/mL and 312.5 $\mu$g/mL, respectively. Their antimicrobial activities in ethanolic extracts were significantly higher than in hot water extracts. In the various solvent fractions prepared from ethanol extract, the ethyl acetate fraction of Achyranthis Radix and the CHCl$_3$ fraction of Moutan radicis Cortexas showed strongest activity.

50가지 생약재를 95% 에탄올과 물추출하여 식중독 세균인 S. aureus와 E. coli O157:H7에 대한 항균활성을 조사하였다. 한천배지확산법에 따른 생육저해효과를 측한 결과, S. aureus 에 대하여 우슬>목단피>지실>황백, 건강, 민들레 순으로, E. coli O157:H7에 대하여 목단피＞우슬>건강>지실, 황백 순으로 활성을 나타내었다. MIC는 S. aureus에 대하여 우슬이 156.25 $\mu$g/mL, 목단피와 황백이 625 $\mu$g/mL로 나타났으며, E. coli O157:H7에 대하여 우슬과 목단피의 MIC는 각각 625, 312.5 $\mu$g/mL로 높은 항균력을 나타내었다. 가장 높은 활성을 보인 우슬과 목단피를 용매분획(hexane, CHC1$_3$, ethyl acetate, butanol)하여 한천배지 확산법에 의한 두 균에 대한 생장저해 효과를 측정하였다. 우슬은 ethyl acetate 분획물에서, 목단피는 CHC1$_3$과 ethyl acetate 분획물에서 각각 높은 활성을 나타내었다.

Keywords

References

  1. Foodborn bacteria pathogens Doyle,M.P.
  2. J. Food Hyg. Safety v.16 Biological Characteristics of Staphylococcus aureus isolated from food-born patients in Seoul Park,S.G.;Y.O.Hwang;J.H.Jang;K.M.Lee
  3. Phytochem. v.37 A survey of antifungal compounds from higher plants Grayer,R.J.;J.B.Harbone https://doi.org/10.1016/0031-9422(94)85005-4
  4. Korean J. Food Sci. Technol. v.30 Isolation and identification of antimicrobial active substance from Sophora flavescens Ait Ahn,E.Y.;D.H.Shin;N.I.Baek;J.A.Oh
  5. Advances in Applied Microbiology v.2 The microbiology of the gens egg Board,R.G.;D.Perlman(ed.)
  6. Korean J. Food Sci. Technol. v.33 Antimicrobial activity of Quercus mongolica leaf ethanol extract and organic acids against food-born microorganisms Kong,Y.J.;B.K.Park;D.H.Oh
  7. Food Technol. v.43 Antimicrobial occurring naturally in foods Beuchat,L.R.;D.A.Golden
  8. J. Nat. Prod. v.58 Antimicrobial metabolites from a bacteria symbiont Li,J.;G.Chen;J.M.Webster https://doi.org/10.1021/np50121a016
  9. Lett. Appl. Microbial. v.26 Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens Smith-Palmer,A.;J.Stewart;L.Fype https://doi.org/10.1046/j.1472-765X.1998.00303.x
  10. J. Food Microbiol. v.56 Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds Rauha J.P. https://doi.org/10.1016/S0168-1605(00)00218-X
  11. Bon-Cho-Kang-Mok Li,S.Z.
  12. Chinese medicine dictionary Lee,K.S.;D.K.Ahn;M.K.Shin;C.M.Kim
  13. Food Technol. v.43 Methods for testing the efficacy of food antimicrobials Gavidson,P.H.;M.E.Parish
  14. Korean J. Food Sci. Technol. v.29 Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates againt foodborn bacteria Shin,D.H.;M.S.Kim;J.S.Han
  15. J. Korean Agric. Chem. Biotechnol. v.36 Antioxidants of Theae Folium and Moutan Cortex Boo,Y.C.;C.O.Jeon
  16. Syoyakugakuzasshi v.20 The occurrence of paeoniflorin in the plant of Paeonia spp. Shibata,S.M.Inba;N.Aimi
  17. Korean J. Postharvest Sci. Technol. v.5 Animicrobial and antioxidative activities from Moutan Cortex extract Kweon,O.G.;J.C.Son;S.C.Kim;S.K.Chung;S.W.Park
  18. Kor. J. Soc. Food Sci. v.14 Screening of antimicrobial activity of the Dandelion(Taraxacum platycarpum) extract Kim,K.H.;H.J.Chun;Y.S.Han
  19. Kor. J. Pharmacogn. v.30 Isolation of Antimicrobial Components from Moutan Cortex Kwon,O.G.;S.H.Kim;B.Y.Chun;C.K.Park;K.H.Son
  20. Planta Med. v.60 Glucuronide saponins of oleanolic acid from Achyranthes fauriei roots Ida,Y.M.Katsumata;Y.Satoh;J.Shoji https://doi.org/10.1055/s-2006-959481
  21. Yakugaku Zasshi v.87 Studies on the constituents of Achyranthis radixⅡ. Isolation of the insectrnoulting hormones Takemoto,T.;S.Ogawa;N.Nishimoto https://doi.org/10.1248/yakushi1947.87.12_1469
  22. Shoyakugaku Zasshi v.35 Plant growth regulating acivities of crude drugs and medicinal plants Shimomura,H.;Y.Sashida;H.Nakata
  23. Shoyakugaku Zasshi v.36 Liver-Protective drugs. 3. The validity of the oriental medicines. 38. Application of carbon teterachloride-induced liver lesion in mice for screening of liver protective crude drug Kiso,Y.;Y.Suzuki;C.Konno;H.Hikino;I.Hashimoto;Y.Yagi