DOI QR코드

DOI QR Code

IN VIVO QUANTITATIVE ANALYSIS OF REMINERALIZATlON EFFECT OF REMINERALIZATlON SOLUTION

구강내에서 재광화용액

  • Kim, Myung-Eun (Department of Conservative Dentistry, college of dentistry, Yonsei University) ;
  • Jung, Il-Young (Department of Conservative Dentistry, college of dentistry, Yonsei University) ;
  • Kum, Kee-Yeon (Department of Conservative Dentistry, college of dentistry, Yonsei University) ;
  • Lee, Chang-Young (Department of Conservative Dentistry, college of dentistry, Yonsei University) ;
  • Roh, Byoung-Duck (Department of Conservative Dentistry, college of dentistry, Yonsei University)
  • 김명은 (연세대학교 치과대학 보존학교실) ;
  • 정영일 (연세대학교 치과대학 보존학교실) ;
  • 금기연 (연세대학교 치과대학 보존학교실) ;
  • 이찬영 (연세대학교 치과대학 보존학교실) ;
  • 노병덕 (연세대학교 치과대학 보존학교실)
  • Published : 2002.03.01

Abstract

Dental caries is a chronic disease that causes the destruction of tooth structure by the interaction of plaque bacteria, food debris, and saliva. There has been attempts to induce remineralization by supersaturating the Intra-oral environment around the surface enamel, where there is incipient caries. In this study, supersaturated remineralized solution "R" was applied to specimens with incipient enamel caries, and the quantitative analysis of remineralization was evaluated using microradiography. Thirty subjects volunteered to participate in this study. Removable appliances were constructed for the subjects, and the enamel specimen with incipient caries were embedded in the appliances. The subjects wore the intra-oral appliance for 15 days except while eating and sleeping. The removable appliance were soaked in supersaturated solution "R", saline, or Senstime$^{\circledR}$ to expose the specimen to those solutions three times a day, 5 minutes each time. After 15 days, microradiography was retaken to compare and evaluate remineralization The results were as the following: 1. The ratio of remineralized area to demineralized area was significantly higher in the supersaturated solution "R" and Senstime$^{\circledR}$ than in the saline (p<0.05) 2. Remineralization in the supersaturated buffer solution "R" occurred in the significantly deeper parts of the tooth. compared to the Senstime$^{\circledR}$ group containing high concentration or fluoride. (p<0.05) As in the above results, the remineralization effect of remineralized buffer solution "R" on incipient enamel caries has been proven. For clinical utilization, further studies on soft tissue reaction and the effect on dentin and cementum are necessary In conclusion compared to commercially available fluoride solution. remineralization solution“R”showed better remineralization effect on early enamel caries lesion, so it is considered as effecient solution for clinical application.

Keywords

References

  1. Arends J., Shuthof J., Jongebloed WL: Lesion depth and microhardness indentations on artificial white spot lesions., Caries Res., 14:190-195. 1980 https://doi.org/10.1159/000260453
  2. Backer Dirks O. Posteruptive changes in dental enamel., J. Dent. Res., Sup.3, 45:503-511, 1966 https://doi.org/10.1177/00220345660450031101
  3. Brudevold F, McCann HG, Gron P in Wolstenholm. Caries resistant teeth as related to the chemistry of enamel .121Churchill, London. 1965
  4. Chow, L. C., and Takagi, S.: Remineralization of Root Lesions with Concentrated Calcium and Phosphate Silutions., Dent. Mat. J.,14:31-36, 1995 https://doi.org/10.4012/dmj.14.31
  5. Clarkson BH, Krell D, Wefel JS, Crall J, Feagin FF : In vitro caries-like lesion produced by Streptococcus mutans and Actinomyces viscosus using sucrose and starch., J. Dent. Res., 66: 795-798, 1987 https://doi.org/10.1177/00220345870660031801
  6. Dawes C and Weatherell JA : Kinetics of fluoride in the oral fluids., J. Dent .Res., 69;638, 1990 https://doi.org/10.1177/00220345900690S125
  7. Exterkate. R.A.M., Damen, J.J.M. and Ten Cate, J. M. : A single-section model for enamel de- and remineralization suties. 1. The effects of different Ca/P ratios in Remineralization solutions., J. Dent. Res., 72: 1599-1603, 1993 https://doi.org/10.1177/00220345930720121201
  8. Hay Dl,Smith DJ, Schluckebier , Moreno EC Relationship between concentration of human salivary statherin and inhibition of calcium phosphate precipitation in stimulated human parotid saliva., J. Dent. Res., 63:857-63,1984 https://doi.org/10.1177/00220345840630060901
  9. Heilman, J. R., and Wefel, J. S.: Effect of remineralization on demineralized root surfaces., J, Dent. Res., 68:351. 1989
  10. Herkstr ter F. M., Noordmans J.. ten Bosch J.J.: Wavelength-independent microradiography: Its use to measure mineral changes in curved and thick samples., Caries Res., 24:399, 1990
  11. Johnson M, Richardson CF, Bergey EJ, Levine MJ, Nancollas GH. The effect of human salivary crystatins and statherin on hydroxyapatite crystallization., Arch oral BioI., 23:993-6,1978 https://doi.org/10.1016/0003-9969(78)90255-8
  12. Koulourdes T. Implication of remineraliztion in the tratment of dental caries in processing symposium on current topics in dental caries in commemoration of the decenial anniversary of Hihon university school of dentistry at Matsudo, Matsuda, Japan. 198.1982
  13. Larsen MJ.,Cemically induced in vitro lesions in dental enamel., Scand. J. Dental. Reserch., 82:496-509, 1974
  14. Larsen MJ. Degrees of saturation with respect to apatite in parotid saliva at various pH values., Scand. J. Dent. Res., 83:7-12.1975
  15. Margolis HC, Murphy BJ, Moreno EC : Development of caries-like lesions in partially saturated lactate buffers. Caries Res., 19: 36-45, 1985 https://doi.org/10.1159/000260827
  16. Margolis HC, Moreno EC, Murphy BJ. Effect fo low levels of fluoride in solution on enamel deminalization., J. Dent. Res., 65(1):23-29,1986 https://doi.org/10.1177/00220345860650010301
  17. Mellberg JG, Ripa LW, and Leske GS : Fluoride in preventive dentistry, theory and clinical application., Quintessence, 151-179, 1983
  18. Moreno, E. C. and Zahradnik, R. T.: Chemistry of enamel subsurface demineralization in vitro., J. Dent. Res., 53:226-235, 1974 https://doi.org/10.1177/00220345740530020901
  19. Moreno EC, Zahradnik RT : Chemistry of enamel subsurface demineralization in vitro., J. Dent. Res., 53:226-235, 1974 https://doi.org/10.1177/00220345740530020901
  20. Ogaard B, Rolla G, and Helgeland K : Alkali soluble and alkali insoluble fluoride retention in demineralized enamel in vivo., Scand J. Dent. Res., 91: 200, 1983
  21. Silverstone LM. Remineralization and enamel caries new concepts., Dent. update.10,1983
  22. Silverstone, L. M.: Observations on the dark zone in early enamel caries and artificial caries-like lesions., Caries Res., 1:261-274, 1967 https://doi.org/10.1159/000259521
  23. Silverstone, L. M.: Structure of carious enamel, including the early lesion., Oral Sci Rev., 3:100-160, 1973
  24. Sperber GH,Bunnocore MG. Enamel surface in whitw spot formation. J. Dent. Res., 42:724-731,1963 https://doi.org/10.1177/00220345630420022101
  25. Ten Bosch J.J., van der Mei H.C., Borsboom P.C.F.: Optical monitor of in vitro caries., Caries Res., 18: 540-548, 1984
  26. Ten Cate JM, Jongebloed WL, Arends J. Remineralization of artificial enamel lesions in vitro IV .Influence of and diphonates in short and long term remineralization., Caries Res., 15:60-69.1981 https://doi.org/10.1159/000260501
  27. Ten Cate JM, Arends J. Remineralization of artificial enamel lesions in vitroIII. A study of the deposition mechanism., Caries Res., 14:351-358,1980 https://doi.org/10.1159/000260477
  28. Ten Cate JM, Arends J. Remineralization of artificial enamel lesions in vitro. Caries Res., 11:277-286.1977 https://doi.org/10.1159/000260279
  29. Theuns, H. M., Van Dijk, J. W. E., Driessens, F. C. M., and Groeneveld, A.: Effect of time, degree of saturation, pH and acid concentration of buffer solutions on the rate of in-vitro demineralization of human enamel., Archs Oral BioI.. 30:37-42. 1985 https://doi.org/10.1016/0003-9969(85)90022-6
  30. A Thylstrup and O. Fejershov:Textbook of clinical cariology.Second edition,231.1994
  31. A Thylstrup and O. Fejershov:Textbook of clinical cariology.Second edition,240,1994
  32. Wefel JS : Effects of fluorides on careis development and progression using intraoral models., J. Dent. Res., 69:626, 1990
  33. Wei, SHY : Clinical uses of fluorides. Lea & Febiger, 16-24,1985
  34. 금기연, 이찬영: 수종의 유기산이 법랑질 인공우식의 형성에 미치는 영향., 대한치과보존학회지, 21: 470-488, 1996
  35. 김민경, 금기연, 이찬영: 법랑질 인공우식의 재광화에 미치는 pH의 영향에 관한 연구., 대한치과보존학회지, 22: 193-208, 1997
  36. 김소라, 이찬영, 금기연:pH 순환모델에서 과포화 용액의 초기우식 법랑질에 대한 재광화 효과., 대한치과보존학회지, 26:341-349,2001
  37. 김종배, 최유진, 백대일, 신승철, 김동기 : 임상예방치학, 이우출판사, 193-218, 1991
  38. 박성호, 이찬영, 이정석: 유산완충액을 이용한 인공치아우식의형성에 미치는 산의 농도와 pH에 관한 연구., 대한치과보존학회지, 18: 277-290,1993
  39. 박정원, 허복, 이찬영 : 유기산 완충용액의 포화도가 법랑질및 상아질의 재광화에 미치는 영향과 산회인회석의 AFM관찰., 대한치과보존학회지,25:459-473,2000
  40. 오현석, 금기연, 노병덕, 이찬영: 산 완충용액의 pH가 인공치근 우식의 형성에 미치는 영향., 대한치과보존학회지, 24:495-502.1999
  41. 이찬영 : 산 완충용액을 이용한 인공치아우식 형성., 연세치대논문집, 7: 34-41, 1992
  42. 한원섭, 금기연 이찬영: 인공치아우식의 재광화에 미치는 불소의 영향., 대한치과보존학회지, 21: 161-173, 1996

Cited by

  1. Changes in surface content and crystal structure after fluoride gel or hydroxyapatite paste application on stripped enamel vol.38, pp.6, 2008, https://doi.org/10.4041/kjod.2008.38.6.407
  2. Color and hardness changes in artificial white spot lesions after resin infiltration vol.37, pp.2, 2012, https://doi.org/10.5395/rde.2012.37.2.90