KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Effects of Planting Date and Accelerated Aging on Seed Germination-related Traits of Lipoxygenase-Lacking Soybean

Lipoxygenase가 결여된 콩의 파종기 및 노화처리에 따른 종실특성과 발아력

  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Lower field germination was observed in lipoxygenase- lacking soybean genotypes. This study was performed to understand seed-related traits after accelerated aging in response to planting date of soybeans. Two soybean cultivars, Jinpumkong (null lipoxygenase-2,3) and Jinpumkong 2 (lacking lipoxygenase-1, 2, 3) were evaluated for germination, and were compared with the cultivar, Taekwangkong containing lipoxygenase-1,2,3 isozymes. Greater seed coat cracking was shown in Jinpumkong and Jinpumkong 2 than Taekwangkong. Regardless of soybean genotypes, earlier planting resulted in greater seed coat cracking. After accelerated aging, seed fracturability and hardness of Jinpumkong and Jinpumkong 2 were lower than those of Taekwangkong. There was significant difference in germination percentage among soybean genotypes in response to planting date. Seeds obtained from late planting showed better germination ability. Accelerated aging resulted in lower germination percentages of Jinpumkong and Jinpumkong 2 than that of Taekwangkong, and showed higher electric conductivity in Jinpumkong and Jinpumkong 2 than Taekwangkong. After accelerated aging at 4$0^{\circ}C$ for thirty five days, germination percentages of Jinpumkong and Jinpumkong 2 were lower than that of Taekwangkong. Electric conductivity was increased continually as accelerated aging time became longer, and Jinpumkong and Jinpumkong 2 showed higher electric conductivity than Taekwangkong. Even though there were significant genotype differences in seed traits, further studies are needed to determine whether seed lipoxygenase ability is associated with germination ability.

Lipoxygenase가 결여된 진품콩(Lx1Lx1lx2lx2lx3lx3), 진품콩 2호(lx1lx1lx2lx2lx3lx3)와 L-1, L-2, L-3이 모두 존재하는 태광콩(Lx1Lx1Lx2Lx2Lx3Lx3)에 대한 종실특성과 인위노화 처리가 종실의 발아율에 미치는 영향을 알아보기 위하여 실시하였다. 1. 종피균열도는 3품종 모두 파종시기가 늦어짐에 따라 감소하였으며, 진품콩과 진품콩 2호가 태광콩보다 높았다. 2. 파쇄성과 경도는 진품콩과 진품콩 2호가 태광콩보다 낮아지는 경향을 나타내었다. 3. 발아율은 진품콩과 진품콩 2호가 태광콩보다 낮았고, 전기전도도는 인위노화 처리전보다 증가하였으며 태광콩, 진품콩, 진품콩 2호 순으로 높았다. 4. 발아율은 인위노화 처리기간이 길어짐에 따라 진품콩과 진품콩 2호가 태광콩보다 낮았으며, 전기전도도는 인위노화 처리기간이 길어짐에 따라 증가하였으며 진품콩과 진품콩 2호가 태광콩보다 높게 나타났다. 인위노화 처리 35일째는 태광콩과 진품콩이 비슷하게 나타났으며, 진품콩 2호는 태광콩과 진품콩보다 높게 나타났다. 5. 종피균열도는 발아율 및 인위노화 처리 후 발아율과는 유의적인 부의 상관을 나타내었다.

Keywords

References

  1. Association of official seed analysts (AOSA). 1983. Seed vigor testing handbook, p 24, 65-70
  2. Bewley, J. D. 1986. Membrane changes in seed as related to germina-tion and the perturbations resulting from deterioration in storage, p. 27-45. In M.B. Macdonald, Jr., and C.J. Ne1son(ed.) Physiology of seed deterioration. CSSA Spec. Publ. 11. Cssa, Madison, WI
  3. Dhindsa, R. S., P. P. Dhindsa, and D. M. Reid. 1982. Leaf senescence and lipid peroxidation : effects of some phytochromes and Scaven-gers of free radicals and singlet oxygen. Physioㅣ Plant. 56 : 453-457 https://doi.org/10.1111/j.1399-3054.1982.tb04539.x
  4. Kim, S. D., Y. H. Kim, K. Y. Park, H. T. Yun, Y. H. Lee, S. H. Lee, Y. K. Seung, H. S. Kim, E. H. Hong and Y. S. Kim. 1997. A new tasteless soybean variety 'Jinpumkong2' witli good quality. RDA J. Agri. Sci. 39(2): 112-115
  5. Kim, S. D., E. H. Hong, Y. H. Kim, S. H. Lee, K, Y. Park, H. T. Yun, Y. K. Seung and H. S. Kim. 1996. A good seed quality beany taste-less soybean variety 'Jinpumkong'. RDA. J. Agri. Sci. 38(2): 166-170
  6. Liu, H. L. 1949. Inheritance of defective seed coat in soybeans. J. Hered.W:W-322
  7. PfeiSFer, T. W., D. F. Hildebrand, and D. M. TeKrony. 1992. Agro-nomic performance of soybean lipoxyeenase isolines. Crop Sci. 32 : 357-362 https://doi.org/10.2135/cropsci1992.0011183X003200020016x
  8. Roberts, E. H. 1973. Predicting the storage life of seeds. Seed Sci. Technol. 1 : 499-514
  9. Stanway, V. 1974. Some factors affecting the cooking quality of the peas and Great Northem type of dry beans. Proceeding of Associa-tion of Official Seed Analysts. 64 : 97-101
  10. Suzuki, M., E. Takahashi, and H. Miyakawa. 1979. Charactehstic of seed coat cracking of soybean and its difference by climatic Condi-tion. Tohoku Aeric. Res. 25. 59-60 [In Japanese]
  11. Tmwatha, S. E., D. M. TeKrony, and D. F. Hildebrand. 1995. Relation-ship of soybean seed quality to fatty acid and $C_6$-aldehyde levels during storage. Crop Sci. 35 : 1415-1422 https://doi.org/10.2135/cropsci1995.0011183X003500050026x
  12. Vick, B. A. and D. C. Zimmerman. 1987. Oxidative systems for modi-fication of fatty acids : The lipoxygenase pathway. The biochemis-try of plants. Vol 9. Academic press. pp. 53-90
  13. Wang J., K. Fujimoto, T. Miyazawa, Y. Endo, and K. Kitamura. 1990. Sensitivities of lipoxygenase-lacking soybean seeds to accelerated aging and their chemiluminescence levels. Phytochemistry. 29 : 3739-3742 https://doi.org/10.1016/0031-9422(90)85323-8