Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Utilization of lacZ to Isolate Regulatory Genes from Corynebacterium glutamicum

  • KIM, HYUNG-JOON (Graduate School of Biotechnology, Korea University) ;
  • JOON-SUNG PARK (Graduate School of Biotechnology, Korea University) ;
  • HEUNG-SHICK LEE (Graduate School of Biotechnology, Korea University) ;
  • YOUNHEE KIM (Department of Oriental Medicine, Semyung University)
  • Published : 2002.04.01
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Abstract

A total of 100 Corynebacterial clones exerting a regulatory effect on the aceB promoter of Corynebacterium glutamicum were isolated by utilizing a reporter carrying the enteric lacZ gene fused to the promoter. The isolated clones were classified into 3 groups of A, B, and C, according to their color of colonies. Escherichia coli cells carrying clones in groups A and B showed a $90\%\;and\;50\%$ reduction in ${\beta}$-galactosidase activity, respectively. The introduction of group A clones into C. glutamicum also resulted in an almost complete reduction in the expression of the aceA and aceB genes, suggesting that the clones express repressor-like proteins for the genes. Although white colonies were formed on plates containing X-gal, E. coli cells carrying one of the clones in group C exhibited intact ${\beta}$-galactosidase activity. The result suggests that the clone may encode proteins that prevent the cells from accumulating the chromogenic compound, X-gal.

Keywords

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