Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Dissolved Oxygen Concentration and pH on the Mass Production of High Molecular Weight Pullulan by Aureobasidium pullulans

  • LEE, JI-HYUN (Division of Food and Biotechnology, Pukyong National University) ;
  • JEONG-HWA KIM (Division of Food and Biotechnology, Pukyong National University) ;
  • MI-RYUNG KIM (Division of Food and Biotechnology, Pukyong National University) ;
  • SUNG-MI LIM (Division of Food and Biotechnology, Pukyong National University) ;
  • SOO-WAN NAM (Department of Microbiology, Dong-Eui University) ;
  • JIN-WOO LEE (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • SUNG-KOO KIM (Division of Food and Biotechnology, Pukyong National University)
  • Published : 2002.02.01
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Abstract

The effects of DO and pH on the mass production of pullulan with high molecular weight and the morphology of A. pullulans ATCC 42023 were evaluated. A. pullulans showed a maximum production of pullulan (11.98 g/l) when the initial pH of the culture broth was 6.5 in a shake-flask culture. In a batch culture, the mixture of a yeast-like and mycelial cell forms was found at a pH of 4.5, and the maximum production of pullulan (13.31 g/l) was obtained. However, a high proportion of high molecular weight pullulan (M.W.>2,000,000) was produced at a pH of 6.5, with a yeast-like morphology. The maximum pullulan production yield ($51\%$) was obtained at a pH noncontrol (initial pH 6.5) and DO control (above $50\%$) condition. Pullulan degrading enzyme was activated when the pH of the broth was lower than 5.0 and the portion of low molecular weight pullulan was increased. The formation of a black pigment was observed at an initial stationary phase, at 40 h of fermentation. Therefore, the fermentation should be carried out in a pH noncontrol (initial pH of 6.5) and DO control (above $50\%$) condition, and should be harvested before reaching the stationary phase (around 40 h) for the production of high molecular weight pullulan.

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References

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