Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지
DOI QR코드

DOI QR Code

Optimal Culture Conditions for Production of Environment-Friendly Biosurfactant by Pseudomonas sp. EL-G527

Pseudomonas sp. EL-G527에 의한 환경친화성 생물계면활성제의 생산최적조건

  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH$_4$NO$_3$, 0.3% KH$_2$PO$_4$, 0.3% $K_2$HPO$_4$, 0.02% MgSO$_4$ㆍ7$H_2O$, 0.0025% CaCk$_2$ㆍ6$H_2O$, 0.0015% FeSO$_4$ㆍ7$H_2O$ in 1$\ell$ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 $m\ell$ of the same medium in 250 $m\ell$ flask. They were cultivated at 3$0^{\circ}C$ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.

Keywords

References

  1. Suzuki, T., K. Tanaka, I. Matsubara and S. Kinoshi, 1969, Trehaloes lipid and ${\alpha}-branched-{\beta}-hydroxyfatty$ acid formed by bacteria grown on n-alkanes, Agr. Biol. Chem., 11, 1619-1627
  2. Zajic, J.E. and K. Seffens, 1984, Biosurfactants, CRC Crit. Rev. Microbial., 5, 87-107
  3. Haferburg, D., R. Hommel, R. Claus and H.P. Kleber, 1986, Extracellura microbial lipids as biosurfactant, Adv. Bioche. Engin/Biotech., 33, 53-53 https://doi.org/10.1007/BFb0002453
  4. Parkinson, M., 1985, Biosurfactants, Biotech. Advs., 3, 65-68 https://doi.org/10.1016/0734-9750(85)90006-0
  5. Zajic, J.E., 1976, Bio-emulsifiers, CRC Crit. Rev. Microbiol., Nov., 39-66
  6. Thomas R., 1996, Sigruficance of bacterial surface-active compounds interaction of bacteria with interface, Microbiol. Rev., 60, 151-166
  7. Kosaric, N., N. C. C. Gray, and W. L. Caims, 1987, Biotechnology and surfactant Industry, Biosurfactants and Biotechnology, N. Kosaric, W. L. Cairns, N. C. C. Gray eds., 1-45
  8. Dunvnjak, J. D. G. Cooper and N. Kosaric, 1982, Production of biosurfactant by Arthrobacter panaffineus ATCC 19558, Biotechnol. Bioeng., 24, 165-175 https://doi.org/10.1002/bit.260240114
  9. Poremba, K., W. Gunkel, S. Lang, and F. Wagner, 1991, Marine biosurfactants, III. toxicity testing with marine microorganisms and comparison with synthetic surfactants, Z. Naturforch, 46c, 21-216 14
  10. Shepherd, R., J. Rockey, I. W. Sutherland, arid S. Roller, 1995, Novel Bioemulsifier from Microorganisms for Use in Foods, J. Biotechnol., 40, 207-217 https://doi.org/10.1016/0168-1656(95)00053-S
  11. Roller, S. and I. C. M. Dea, 1992, Biotechnology in the Production and Modification of Biopolymers for Foods, CRC Crit. Rev. Biotechnol., 12, 261-277 https://doi.org/10.3109/07388559209069195
  12. Park, J.Y. and I.S. Park, 1998, Emulsification of bunker-C oil by a marine bacterium Arthrobacter sp. M-1220, Kor. J. Appl. Microbiol. Bioeng., 16, 384-388
  13. Gutnick, D.L. and E. Rosenberg, 1977, Oil tankers and pollution: a microbiological approach, Ann Rev. Microbiol, 31, 379-396 https://doi.org/10.1146/annurev.mi.31.100177.002115
  14. Aheam, G.P., 1987, Surfactants for oi1 recovery, J. Am. Oil Chem. Soc., 46, 540-580 https://doi.org/10.1007/BF02633160
  15. Beersteacher, E., 1954, Petroleum Microbiology, Elsevier, Houston
  16. Kim, D.W., M.J. Kim and S.M. Kang, 1995, Purification and properties of Biosurfactant from Pseudomonas aeruginosa, Kor. J. Appl. Microbiol. Biotechnol., 23, 337-345
  17. Lee, S.J., 1993, Characteristics of the rhamnolipid biosurfactant prepared with Pseudomonas sp, 13, Ph. D. Thesis, Dept. of Chem. Eng., Chun Buk National Univ, Chunjoo
  18. Lee, K.H., 1993, Carbon mass balance analysis of biosurfactant, sophorose lipid, fermentation by Torulopsis bombicola, Ph. D. Thesis, Dept. of Biotechnology, KAIST, Daejon
  19. Lee, J.M., 1993, The studies on biosurfactant production by Microorganism, M. S. Thesis, Dept. of Microbiology, Pusan National Univ., Busan
  20. Chopineau, J., F.D. MacCafferty, M. Therisod, and A.M. Klibanov, 1988, Production of biosurfactant from sugar alcohol and vegetable oils catalyzed by lipase in a nonaqueous medium， Biotechno1. Bioeng., 31, 208-214 https://doi.org/10.1002/bit.260310305
  21. Magaritis, A., K. Kennedy, J,E. Jajic, and D.F. Gerson, 1979, Biosurfactant production by Nocardia erythropolis, Develp. Ind, Microbiol., 20, 623-630
  22. Luis, G.S. and K. Othmar, 1984, Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source, Appl. Environ. Microbiol., 48, 301-305
  23. Krieg, N.R. and J.G. Holt, 1984, Bergey's Manual of Systematic Bacteriology, 1, The William and Wilkins Co., U.S.A
  24. Krieg, N.R. and J.G. Holt. 1974, Bergey's Manual of Determinative Bacteriology 9th ed. The William and Wilkins Co., U.S.A, 21
  25. Itoh, S. and T. Suzuki, 1974, Sucrose lipids of Arthrobacter, Corγnebacteria， and Nocardia grown on sucrose， Agri. Biol. Chem, 38, 567-563
  26. Hisatsuka, K., 1971, Formation of rhamnolipids by Pseudomonas aeuruginosa and it's function in hydrocarbon fermentation, Agri. Biol. Chem., 35, 686-692 https://doi.org/10.1271/bbb1961.35.686