Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Analysis of Melanocortin Receptor 1 (MC1R) Genotype in Korean Brindle Cattle and Korean Cattle with Dark Muzzle

칡소와 비경흑색 한우의 Melanocortin Receptor 1 (MC1R) 유전자형 분석

  • Lee, S.S. (National Jeju Agricultural Experiment Station) ;
  • Yang, B.S. (National Jeju Agricultural Experiment Station) ;
  • Yang, Y.H. (Department of Animal Biotechnology, Cheju National University) ;
  • Gang, S.Y. (National Jeju Agricultural Experiment Station) ;
  • Ko, S.B. (National Jeju Agricultural Experiment Station) ;
  • Jeong, J.K. (National Jeju Agricultural Experiment Station) ;
  • Oh, W.Y. (National Livestock Research Institute) ;
  • Oh, S.J. (National Livestock Research Institute) ;
  • Kim, K.I. (Department of Animal Biotechnology, Cheju National University)
  • 이성수 (농촌진흥청 제주농업시험장) ;
  • 양보석 (농촌진흥청 제주농업시험장) ;
  • 양영훈 (제주대학교 동물자원과학과) ;
  • 강승률 (농촌진흥청 제주농업시험장) ;
  • 고성봉 (농촌진흥청 제주농업시험장) ;
  • 정진관 (농촌진흥청 제주농업시험장) ;
  • 오운용 (농촌진흥청 축산기술연구소) ;
  • 오성종 (농촌진흥청 축산기술연구소) ;
  • 김규일 (제주대학교 동물자원과학과)
  • Published : 2002.02.28
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Abstract

PCR-RFLP analysis was carried out to investigate the genotype of Melanocortin receptor 1 (MC1R) gene in Korean Brindle Cattle and Korean Cattle with dark muzzle, which are coat color and muzzle pigmentation variants of Korean Cattle, respectively. Allelic variants of MC1R in cattle were analyzed by digestion with BsrFⅠ, AciⅠ. Among six genotypes, $E^D/E^D,\;E^D/E^+,\;E^D/e,\;E^+/E^+,\;E^+$/e and e/e, detected in cattle, only two genotypes, $E^+/E^+\;and\;E^+$/e, were observed in Korean Brindle Cattle, probably reflecting the necessary of $E^+$ allele for the expression of black brindle coat color. As in Korean Cattle with light muzzle, the $E^+$/e and e/e genotypes were detected in Korean Cattle with dark muzzle. The $E^+$ and e alleles frequencies in two populations of Korean Cattle with dark muzzle and with light muzzle were 0.37, 0.63 and 0,11, 0.89, respectively. Although the frequency of $E^+$ allele in Korean Cattle with dark muzzle was higher than in Korean Cattle with light muzzle, the $E^+$ allele was not completely associated with dark muzzle pigmentation. The results of this experiment indicate that the difference of MC1R genotype and frequency may be useful for fixation of coat color in Korean Cattle as well as Korean Brindle Cattle.

칡소와 비경흑색 한우의 MC1R 유전자의 유전자형를 조사하고 칡소의 모색 발현과 한우 비경색과의 관계를 규명하기 위하여 MC1R 유전자의 PCR-RFLP 분석을 수행하였다. 소에서 나타나는 6가지 유전자형($E^D/E^D,\;E^D/E^+,\;E^D/e,\;E^+/E^+,\;E^+$/e와 e/e) 중 칡소에서는 단지 2개의 유전자형 $E^+/E^+$와 $E^+$/e 만이 출현하였고, e/e 유전자형을 가지는 개체는 전혀 출현하지 않아 제주재래흑우에서와 같이 흑색 호반모가 발현되기 위해서는 기본적으로 $E^+$ allele이 필요한 것으로 사료되었다. 비경흑색 한우와 비경황색 한우에서는 모두 $E^+$/e 혹은 e/e 유전자형이 출현하였고 $E^+$와 e allele의 빈도는 각각 0.37, 0.63과 0.11, 0.89였다. 비록 $E^+$ allele의 빈도가 비경흑색 한우에서 비경황색 한우에서 보다 높았지만, $E^+$ allele과 비경 흑색과는 완전한 연관성은 없었다. 이러한 결과는 MC1R 유전자 분석이 칡소 뿐만 아니라 한우의 모색 고정에도 유용한 도구로 이용 가능함을 제시하고 있다.

Keywords

References

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