Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Cold Storage of Milt from Four Species of Flatfish

  • Chang Yun Jeong (Department of Aquaculture, Pukyong National University) ;
  • Chang Young Jin (Department of Aquaculture, Pukyong National University) ;
  • Lim Han Kyu (Uljin Hatchery, National Fisheries Research and Development Institute) ;
  • Lee Jong Kwan (Uljin Hatchery, National Fisheries Research and Development Institute) ;
  • Park Young Je (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The proper conditions for cold storage of milt in four species of flatfish were investigated. Various diluents were tested for the preservation of marbled sole, brown sole, starry flounder and olive flounder milt at $1\pm0.5^{\circ}C$ for 7 days. The most effective diluent was $1\%$ NaCl for marbled sole and Stein's extender for brown sole, starry flounder and olive flounder. After 7 days of storage in different dilution ratios (milt: diluent), marbled sole milt diluted with 1:3-10 and brown sole, starry flounder and olive flounder milt diluted 1:1-10 maintained good sperm activity index (SAI). SAI was affected by storage volume and presence of antibiotic. SAI was lower after 7 days storage in the volume of 1.5 mL than in 0.5 or 1.0 mL. SAI after 29 days storage was markedly enhanced by addition of antibiotic in marbled sole (400 ppm gentamicin, 400 ppm neomycin, 200-400 ppm streptomycin), brown sole (600 ppm gentamicin), starry flounder (1,000 ppm gentamicin) and olive flounder (200-1,000 ppm gentamicin, 200-400 ppm neomycin, 200-1,000 ppm streptomycin).

Keywords

References

  1. Barrett, I. 1951. Fertility of salmonid eggs and sperm after storage. J. Fish. Res. Bd. Can., 8, 125-133
  2. Chao, N.H., H.P. Chen and I.C. Liao. 1975. Study on Cryogenie preservation of grey mullet sperm. Aquaculture, 5,389-406 https://doi.org/10.1016/0044-8486(75)90058-7
  3. Chao, N.H., H.P. Tsai and I.C. Liao. 1992. Short- and 10ng-term cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Sch-neider). Asian Fish. Sci., 5, 103-116
  4. Erdahl, A.W. and E.F. Graham. 1987. Fertility of teleost semen as affected by dilution and storage in a seminal Plasma-mimicking medium. Aquaculture, 60, 311-321 https://doi.org/10.1016/0044-8486(87)90296-1
  5. Hara, S., J.T. Canto and J.M.E. Almendras. 1982. A Comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28, 339-346 https://doi.org/10.1016/0044-8486(82)90076-X
  6. McNiven, M.A., R.K. Gallant and G.F. Richardson. 1993. Fresh storage of rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109, 71-82 https://doi.org/10.1016/0044-8486(93)90487-J
  7. Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleosts. Zool. Sci., 2, 605-615
  8. Ohta, H. and T. Izawa. 1996. Diluent for cool storage of Japanese eel (Ansuilla japonica) spermatozoa. Aquaculture, 142, 107-118 https://doi.org/10.1016/0044-8486(95)01246-X
  9. Saad, A., R. Billard, M.C. Theron and M.G. Hollebecq. 1988. Short-term preservation of carp (Cypiinus carpio) semen. Aquaculture, 71, 133-150 https://doi.org/10.1016/0044-8486(88)90280-3
  10. Scott, A.P. and S.M. Baynes. 1980. A review of the biology, handling and storage of salmonid spermatozoa. J. Fish. Biol., 17, 707-739 https://doi.org/10.1111/j.1095-8649.1980.tb02804.x
  11. Stoss, J. 1983. Fish gamete preservation and spermatozoan physiology. Fish Physiol. 9B, 305-350, Academic Press, New York
  12. Stoss, J., S. Buyukhatipoglu and W. Holtz. 1978. Short-term storage and cryopreservation of rainbow trout (Salmogairdneri Richardson) sperm. Ann. Biol. Anim. Biochem. Biophys., 18, 1077-1082 https://doi.org/10.1051/rnd:19780549
  13. Stoss, J. and W. Holtz. 1983. Successful storage of chilled rainbow trout (Salmo gairdneri) spermatozoa for up to 34 days. Aquaculture, 31, 269-274 https://doi.org/10.1016/0044-8486(83)90318-6
  14. Stoss, J. and T. Refstie. 1983. Short-term storage and Cryo-preservation of milt from Atlantic salmon and sea trout. Aquaculture, 30, 229-236 https://doi.org/10.1016/0044-8486(83)90165-5
  15. Stoss, J., L. Geries and W. Holtz. 1987. The role of sample depth in storing chilled rainbow trout (Salmo eairdnen) semen under oxygen. Aquaculture, 61, 275-279 https://doi.org/10.1016/0044-8486(87)90155-4
  16. Truscott, B., D.R. Idler, R.J. Hoyle and H.C. Freeman. 1968. Sub-zero preservation of Atlantic salmon sperm. J. Fish. Res. Bd. Can., 25, 363-372 https://doi.org/10.1139/f68-028
  17. Yao, Z, G.F. Richardson and L.W. Crim. 1999. A diluent for prolonged motility of ocean pout (Macrozoarces ameri-canus L.) sperm. Aquaculture, 174, 183-193 https://doi.org/10.1016/S0044-8486(99)00014-9

Cited by

  1. 희석액벼려 넙치(Paralichthys olivaceus) 정자의 냉장보존 효과 vol.38, pp.4, 2002, https://doi.org/10.5657/kfas.2005.38.4.239
  2. Antibiotics and oxygen availability affect the short-term storage of spermatozoa from the critically endangered booroolong frog, Litoria booroolongensis vol.27, pp.8, 2002, https://doi.org/10.1071/rd14062
  3. Short term cold storage and sperm concentration assessment of lumpfish (Cyclopterus lumpus. L) Milt vol.529, pp.None, 2020, https://doi.org/10.1016/j.aquaculture.2020.735646