Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Studies on the Hydrolysis of Seaweed Using Microorganisms and Its Application -I. Screening of Microfloras Involved in Hydrolysis of Sea Tangle (Laminaria japonica) and Sea Mustard (Undaria pinnatifida)

미생물을 이용한 해조류의 가수분해 및 이용 -I. 다시마와 미역을 가수분해시키는 미생물군의 탐색-

  • Kim Hae Sub (Division of Food Technology and Nutrition, Yosu National University) ;
  • Bae Tae Jin (Division of Food Technology and Nutrition, Yosu National University)
  • 김해섭 (여수대학교 식품공학영양학부) ;
  • 배태진 (여수대학교 식품공학영양학부)
  • Published : 2002.07.01
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Abstract

The purpose of this study is screening of microfloras involved in hydrolysis of sea tangle (Laminaria japonica) and sea mustard ( Undaria pinnatifida), This is a part of studies on the hydrolysis of seaweed using microorganisms. General process is difficult to extract of the useful constituent parts as intercellular mucilage, storage polysaccharide and mineral from seafood. It was screening to thirty-three microflora samples as destructed of tissue in sea tangle and sea mustard to about two hundred microflora samples from mountain, rice field, dry field, sea, seaside and fish market etc. in the neighborhood of Yeosu. Sufficient results of the naked eye observation were obtained at eight microflora samples as a feces of bull, a decayed pine tree, a soil of dry field, the mud of the banks in a rice Held, the water of a ditch in a rice Held, the weed of the banks in a rice field, the water in a rice field and leaved in the air, And the value of chemical analysis of the sample is much better in comparison with control. Accordingly, the hydrolysis of seaweed using microorganisms in the inside of these microflora samples can possibility.

조직이 단단하여 이용률이 낮은 해조류의 조직을 가수분해시키고 유용성분을 효율적으로 추출하기 위하여, 자연계에서 미생물을 탐색하여 해조류의 가수분해에 사용하고자 하였다. 우선 자연계에서 약 200종류의 미생물군 시료를 수집하고, 이중에서 다시마와 미역의 조직파괴가 인정되는 33군의 시료를 사용하여 다시마와 미역의 조직파괴 및 가수분해 가능성에 대하여 검토하였다. 소의 배설물에서 취한 시료 (No. 5), 공중낙하균군 시료 (No.33), 토양에서 취한 시료 (No. 10, 26), 담수에서 취한 시료 (No.27, 30) 및 부식한 식물에서 취한 시료 (No. 8, 28)에서 다시마와 미역의 조체 붕괴가 관찰되었다 그리고 이들 미생물군들을 다시 다시마와 미역에 접종하여 4주간 배양하고, 그 배양물 중의 전당과 환원당 함량, 추출을 및 분해율을 측정한 결과 두 해조 모두에서 시료 번호 27번과 8번이 가수분해에 가장 효과적이었다 이들 미생물군에 의하여 가수분해된 배양물에서 전당의 추출율은 다시마에서 각각 $68.55\%$ 및 $44.02%$이었고, 미역에서는 각각 $80.87\%$ 및 $62.60\%$이었다. 그리고 환원당의 추출율은 다시마에서 각각 $47.59%$ 및 $28.81\%$, 미역에서는 각각 $76.83\%$ 및 $39.01\%$이었다.

Keywords

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