Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effect of Bee Venom & Melittin Solution on Cell Death in Synovial Cell Line

봉독(蜂毒) 및 Melittin 약침액(藥鍼液)이 관절염(關節炎) 활액세포(滑液細胞)에 미치는 영향(影響)

  • Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Park, Ki-hyeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Jung, Tae-young (Je-Han Medical Clinic of Kyung-San University) ;
  • Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University)
  • 한상원 (경산대학교 한의과대학 침구경혈학교실) ;
  • 박기현 (경산대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (경산대학교부속 제한의원) ;
  • 서정철 (경산대학교 한의과대학 침구경혈학교실)
  • Received : 2002.06.25
  • Accepted : 2002.07.14
  • Published : 2002.08.20
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Abstract

Objectives : This study is aimed to investigate the effects of bee venom and melittin on cell death in synovial cell line. Methods : It was evaluated by using MTT assay, morphologic method, DNA fragmenation, NO generation, flow cytometry, immunocytochemistry analysis, RT-PCR, Western blot. Results : The obtained results are summarized as follows: 1. The MTT assay demonstrated that synovial cell viability was significantly inhibitted dose-dependently by treatment with bee venom and melittin in comparison with control. 2. The morphologic study demonstrated that synovial cell showed apoptosis after treatment with bee venom and melittin for 6 hours using microscope. 3. In case of NO generation bee venom group and melittin group showed significant inhibition in comparison with control. 4. The Flow cytometry demonstrated that apoptosis of synovial cell treated with bee venom and melittin was related with stop of cell cycle in stage of $G_0/G_1$. 5. DNA fragmenation demonstrated that synovial cell treated with bee venom and melittin showed DNA ladder below l Kbp. 6. Immunocytochemistry assay demonstrated that COX-II and PLA2 were strongly down-regulated by treatment with bee venom and melittin whereas iNOS was almostly not expressed by bee venom treatment and slightly expressed by melittin treatment. 7. RT-PCR analysis demonstrated that iNOS were strongly down-regulated by treatment with bee venom and melittin whereas COX-II was almostly not expressed by bee venom treatment and slightly expressed by melittin treatment. 8. Western blot demonstrated that iNOS were strongly down-regulated by treatment with $15{\mu}g/ml$ bee venom whereas COX-II was strongly down-regulated from $5{\mu}g/ml$ bee venom. Conclusions : These results suggest that bee venom and melittin have significant effect on cell death in synovial cell line and further study is needed in vivo.

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