Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Evaluation of the Anti-inflammatory and Immunomodulatory Effects of BSASM Using in vitro Experiments

시험관내에서 천연물제제 BSASM의 항염증 및 면역억제 효능 평가

  • 이종성 (바이오스펙트럼 생명과학연구소) ;
  • 박유미 (바이오스펙트럼 생명과학연구소) ;
  • 박병화 (바이오스펙트럼 생명과학연구소) ;
  • 정광선 (바이오스펙트럼 생명과학연구소) ;
  • 김국현 (바이오스펙트럼 생명과학연구소) ;
  • 이원희 (녹십자 R&D) ;
  • 박덕훈 (바이오스펙트럼 생명과학연구소)
  • Published : 2003.09.30
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Abstract

For effective management of atopic dermatitis, it is important to introduce a therapeutic agent although having the fewest side effects, has the greatest anti- inflammatory effect. In the course of screening anti-inflammatory agents, we obtained BSASM composed of several plant extracts. This study was designed to investigate anti-inflammatory and immunomodulatory effects of BSASM. As a first step, $NF-{\kappa}B$ luciferase reporter assay was performed to know the involvement of BSASM in the production of proinflammatory cytokines because $NF-{\kappa}B$ element has been known to play a major role in expression of cytokine genes such as interleukin-8 (IL-8) or tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$. LPS (lipolysaccharide)-induced $NF-{\kappa}B$ activation was inhibited by BSASM. In addition, we found the fact that BSASM inhibits LPS-induced produced production of IL-8 and $TNF-{\alpha}$ proinflammatory cytokines, indicating BSASM has anti-inflammatory effect. In interleukin-2 (IL-2) luciferase reporter assay in Jurkat T cells, BSASM reduced PHA (Phytohemagglutinin)-induced IL-2 luciferase activity, suggesting the possibility that BSASM might also have an immunomodulatory function in T cell-mediated immune response. Based on these results, we suggest the possibility that BSASM can be introduced to improve symptom of immune-related skin diseases, namely, atopic dermatitis.

Keywords

References

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