Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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In vitro Antiinflammatory Activity of Amygdalin in Murine Macrophage Raw 264.7 Cells

Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과

  • Published : 2003.09.30
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Abstract

In the present study, anti-inflammatory activity of amygdalin isolated from persicae Semen have been evaluated on lipopolysaccharide (LPS)-induced release of nitric oxide (NO), prostaglandin $E_2\;(PGE_2)$ and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) by the macrophage RAW 264.7 cells. Amygdalin significantly inhibited generation of NO and $TNF-{\alpha}$ on LPS-stimulated RAW264.7 cells in a concentration-dependent manner. Consistent with these observations, the expression of inducible NO synthase (iNOS) enzyme was also inhibited by amygdalin in a concentration-dependent manner. However, amygdalin did not show any influence on the synthesis of $PGE_2$ and the expression of COX-2. Thus, this study suggests that amygdalin-mediated inhibition of iNOS expression, and $TNF-{\alpha}$ release may be one of the mechanisms responsible for the anti-inflammatory effects of Persicae Semen.

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References

  1. Moncada, S., Palmer, R. M., and Higgs, E. A. (1991) Nitric oxide: physiology. pathophysiology, and pharmacology. Phannacol. Rev. 43: 109-142
  2. Nathan, C. (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J. 6: 3051-3064
  3. Knowles, R. G. and Mocada, S. (1992) Nitric oxide as a signal in blood vessels. TIBS. 17: 399-402
  4. Stuehr, H. H. J., Kwon, N. S., Weise, M., and Nathan, C. (1991) Purification of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein. Proc. Natl. Sci. U. S. A. 88: 7773-7777 https://doi.org/10.1073/pnas.88.17.7773
  5. McCartney-Francis, N., Allen, J. B., Mizel, D. E., Albina, J. E., Xie, Q. W., Nathan, C. F., and Wahl, S. M. (1993) Suppression of arthritis by an inhibitor of nitric oxide synthase. J. Exp. Med. 178: 749-754 https://doi.org/10.1084/jem.178.2.749
  6. Weisz,A., Cicatiello, I., and Esumi, H. (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochem. J. 316: 209-215
  7. Duerksen-Hughes, P. J., Day, D., Laster, S. M. A., Zacharidaes, N. A., Aquino, L., and Gooding, L. R. (1992) Both tumor necrosis factor and nitric oxide participate in lysis of simian virus 40-transformed cells by activated macrophages. J. Immunol. 149: 2114-2122
  8. Willeaume, V., Kruys, V., Mijatovic,T., and Huez, G. (1995) Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J. Inflamm. 46: 1-12
  9. Vane, J. R. (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat. New. Biol. 23: 232-235
  10. 생약학연구회(1997) 현대생약햑, 322. 학창사, 서울
  11. Hwang, E. Y, Lee, S. S., Lee, J. H., and Hong, S. P. (2002) Arch. Pharm. Res. 5: 453-456
  12. Kim, Y. K., Kim, R. G., Park, S. J., Ha, J. H., Choi, J. W., Park H. J., and Lee, K. T. (2002) In vitro antiinflammatory activity of kalopanaxsaponin A isolated from Kalopanax pictus in murine macrophage RAW 264.7 cells. Biol. Pharm. Bull. 25: 472-476 https://doi.org/10.1248/bpb.25.472
  13. Kim, R. G., Shin, K. M., Kim, Y. K., Jeong, H. J., Ha, J., Choi, J. W., Park, H. J., and Lee K. T. (2003) Inhibition of methanol extract from the aerial parts of saururus chinensis on lipopolysaccharide-induced nitric oxide and prostagladin $E_2$ production from murine macrophage RAW 264.7 cells. Biol. Pharm. Bull. 26: 481-486 https://doi.org/10.1248/bpb.26.481
  14. Funk, C. D., Fronk, L. B., Kennedy, M. E., Pong, A. S., and Fitzgerald, G. A. (1991) Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J. 5: 2304-2312
  15. Feldmann, M., Brennan, F. M., and Maini, R. N. (1996) Role of cytokines in rheumatoid arthritis. Annu. Rev. Immunol. 14: 397-440 https://doi.org/10.1146/annurev.immunol.14.1.397
  16. Harada, A., Sekido, N., Akahoshi, T., Wada, T., Mukaida, N., and Matsushima, K. J. (1994) Essential involvement of interleukin-8 (IL-8) in acute inflammation. J. Leukoc. Biol. 56: 559-564
  17. Seibert, K., Zhang, Y., Leahy, K., Hauser, S., Masferrer, J., Perkins, W., Lee, L., and Ksakson, P. (1994) Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc. Natl. Acad. Sci. U.S.A. 91: 12013-12017 https://doi.org/10.1073/pnas.91.25.12013
  18. Masferrer, J., Zweifel B. S., Manning, P. T., Hauser, S. D., Leahy, K. M., Smith, W. G., Isakson, P. C., and Seibert, K. (1994) Selective inhibition of inducible cyclooxygenase 2 in vivo is antiinflammatory and nonulcerogenic. Proc. Natl. Acad. Sci. U.S.A. 91: 3228-3232 https://doi.org/10.1073/pnas.91.8.3228