Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Growth enhancement and cytotoxicity of Korean mistletoe fractions on human cell lines

한국산 겨우살이 분획물의 면역세포의 생육증진 및 세포독성

  • Lee, So-Jin (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Mi-Kyoung (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Geun-Pyo (Dept. Food & Life Science, Gangwon Provincial University) ;
  • Yu, Chang-Yeon (Division of Applied Plant Science, College of Agriculture and Life Science, Kangwon National University) ;
  • Roh, Seong-Kyu (Dept. Physical Education, Kangwon National University) ;
  • Kim, Jong-Dai (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Jin-Ha (School of Biotechnology and Bioengineering, Kangwon National University)
  • 이소진 (강원대학교 바이오산업공학부) ;
  • 이미경 (강원대학교 바이오산업공학부) ;
  • 최근표 (강원전문대학 식품생명과학과) ;
  • 유창연 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 노성규 (강원대학교 체육학부) ;
  • 김종대 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 이진하 (강원대학교 바이오산업공학부)
  • Published : 2003.03.31
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Abstract

The biological activities on human immune and cancer cell lines of the four kinds of Korean mistletoes (Korean Viscum album, var. coloratum, : Korean Viscum sp. in Quercus acutissima Carr., Korean Viscum sp. in Castanea crenata, Korean Viscum sp. in Betula platyphylla, and Korean Viscum sp. in Salix koreensis) extracts were investigated. The extracts were preparated with ethanol, and fractionated with n-butanol, ethyl acetate, chloroform, hexane, and second distilled water. Cytotoxic potencies of the fractions on human normal lung cell line (HEL 299) showed under 28% in the concentration of 0.5 mg/ml. Growth inhibition effect of the Korean mistletoe extracts on the several human cancer cell lines depends on the concentration of the extracts, and extracting solvent. The hexane, chloroform, and ethyl acetate fractions indicated a strong anticancer activity, but not in aqueous and butanol fractions. Some mistletoe fractions have a different characteristic on the cancer cell lines. Stimulation on the growth of human immuno cell lines(B cell : Raji, T cell: Jurkat) of the extracts were confirmed in the ethyl acetate, chloroform, hexane fractions, but not in aqueous system.

기주목(상수리나무, 밤나무, 자작나무, 버드나무)별 겨우살이 ethanol 추출물의 5가지 분획물(hexane fr., chloroform fr., ethyl acetate fr., butanol fr., aqueous fr.)을 이용하여 in vitro에서 사람 면역세포주에 대한 생육활성, 사람 정상 세포주에 대한 세포독성, 사람의 각종 암 세포주에 대한 생육 저해능을 조사하였다. 4종의 한국산 겨우살이 분획물들의 정상세포에 대한 세포독성은 밤나무 겨우살이 chloroform fr.(28%)을 제외한 모든 겨우살이 분획물 0.5mg/ml이하의 농도로 투여시 정상세포에 대한 세포독성이 20%이하로 나타났다. 또한 암세포 생육 저해능의 경우, 폐암 세포주 A549와 위암세포주 AGS에 대하여 상수리나무, 밤나무, 버드나무 겨우살이 분획물에서 80%이상의 생육억제 효과를 보였다. Human B 세포주와 T 세포주에 대한 생육촉진 실험에서, B 세포주에 대하여 상수리나무 겨우살이 hexane fr. buthanol fr., 밤나무 겨우살이 hexane fr., 자작나무 겨우살이 ethyl acetate fr., 버드나무 겨우살이 hexane fr. chloroform fr. ethyl acetate fr.은 배양 3일에서 5일 사이 control에 비하여 약 $1.5{\sim}3$배의 세포 생육촉진을 보였다. T 세포주에 대하여 상수리나무 겨우살이 chloroform fr. ethyl acetate fr., 밤나무 겨우살이 chloroform fr. ethyl acetate fr., 자작나무 겨우살이 hexane fr. chloroform fr. ethyl acetate fr., 버드나무 겨우살이 hexane fr. chloroform fr. ethyl acetate fr.은 배양 3일에서 5일 사이 control에 비하여 약 $1.5{\sim}2.3$배의 세포 생육촉진을 보였다. 한국산 겨우살이 분획물들은 정상세포 대한 독성이 약하고, 면역세포의 생육을 촉진하며, 암세포 생육을 저해하는 효과가 있는 결과로부터 겨우살이는 신체질병에 대한 효과가 기대되며, 앞으로 보다 상세한 연구가 필요할 것으로 사료된다.

Keywords

References

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