Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Inhibitory effect of Korean mistletoes on the oxidative DNA damage

한국산 겨우살이의 산화적 DNA 손상 억제작용

  • Lee, So-Jin (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Mi-Kyoung (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Geun-Pyo (Dept. Food & Life Science, Gangwon Provincial University) ;
  • Kim, Na-Young (Dept. of Food and Nutrition, KyungHee Uni.) ;
  • Roh, Seong-Kyu (Dept. Physical Education, Kangwon National University) ;
  • Heo, Moon-Young (College of Pharmacy, Kangwon National University) ;
  • Kim, Jong-Dai (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Jin-Ha (School of Biotechnology and Bioengineering, Kangwon National University)
  • 이소진 (강원대학교 바이오산업공학부) ;
  • 이미경 (강원대학교 바이오산업공학부) ;
  • 최근표 (강원도립대학 식품생명과학과) ;
  • 김나영 (경희대학교 식품영양학과) ;
  • 노성규 (강원대학교 체육학부) ;
  • 허문영 (강원대학교 약학대학) ;
  • 김종대 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 이진하 (강원대학교 바이오산업공학부)
  • Published : 2003.06.30
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Abstract

Korean mistletoes extracts were investigated for in vitro antioxidation activity, with 1,1-diphenyl-2-picrylhydrazine(DPPH), and an inhibitory effect on oxidative DNA damage by using comet assay. The Korean mistletoes were 4 different kinds classified by their host plants (Korean Viscum sp. in Quercus acutissima Carr., Korean Viscum sp. in Castanea crenata, Korean Viscum sp. in Betula platyphylla, and Korean Viscum sp. in Salix koreensis). The samples were extracted with ethanol, and fractonationed with n-butanol, ethyl acetate, chloroform, n-hexane, and second distilled water. Among them, ethyl acetate fraction from Korean Viscum sp. in Betula platyphylla showed the strongest activities to electron donating ability on 1,1-diphenyl-2-picrylhydrazyl(DPPH) and the protective effect on oxidative DNA damage.

기주목(상수리나무, 밤나무, 자작나무, 버드나무)별 겨우살이 ethanol추출물과 5가지 분획물 (hexane fr., chloroform fr., ethyl acetate fr., butanol fr., aqueous fr.)을 이용하여 in vitro에서 DPPH를 이용한 수소전자공여능, DNA에 대한 산화적 손상 및 손상억제 효과 등에 미치는 영향을 연구하기 위하여 comet assay를 실시하였다. 4종의 한국산 겨우살이의 ethanol추출물과 분획물 중 ethyl acetate fr.들이 높은 수소전자공여능을 보였다. Comet assay분석에서 head DNA의 형태를 유지하거나 또는 tail DNA의 생성을 억제하는 겨우살이 fr.들이 DNA에 대하여 항산화적 기능이 있음을 보여 주었다. 또는 comet assay 유용한 parameter인 $tail\;length({\mu}m)$는 positiye control인 $H_2O_2\;(10^{-3}M)$로 40min동안 세포에 처리하여 산화적 손상을 준 결과, $149{\mu}m$의 tail length를 보였으며 , 상수리나무 겨우살이의 ethyl acetate fr.(0.1mg/ml)을 $H_2O_2\;(10^{-3}M)$와 동시 처리에는 $110{\mu}m$, 자작나무 겨우살이 ethyl acetate fr.은 $104{\mu}m$, 상수리나무 겨우살이 butanol fr.은 $123{\mu}m$, 자작나무 겨우살이 butanol fr.은 $118{\mu}m$로 tail length가 감소하는 것으로 보아 겨우살이가 산화적 DNA손상을 방어한다는 것을 알 수 있었다. 이상의 결과로 볼 때 한국산 겨우살이는 산화적 stress를 일으키는 활성 산소 자유라디칼의 생성에 대해 보호활성을 나타내어 유용한 chemopreventive agent의 기능이 기대된다.

Keywords

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