Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Comparison of biological activities of essential oils from Foeniculum vulgare Mill, Boswellia carteii Birew and Juniperus rigida Sieb. by a supercritical fluid extraction system

초임계 추출 공법을 이용해 회향, 유향 및 노간주나무로부터 분리한 정유 성분의 생리활성 비교

  • Lee, Hyun-Soo (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Mun, Chul-Hyung (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Park, Jin-Hong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Dae-Ho (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Yoo, Jae-Eun (O.Z. Tech) ;
  • Park, Young-Sik (O.Z. Tech) ;
  • Ryu, Lee-Ha (JINRO Ltd.) ;
  • Choi, Keun-Pyo (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
  • 이현수 (강원대학교 바이오산업공학부) ;
  • 문형철 (강원대학교 바이오산업공학부) ;
  • 박진홍 (강원대학교 바이오산업공학부) ;
  • 김대호 (강원대학교 바이오산업공학부) ;
  • 유재은 ((주) 오지텍) ;
  • 박영식 ((주) 오지텍) ;
  • 류이하 ((주) 진로) ;
  • 최근표 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부)
  • Published : 2003.06.30
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Abstract

Essential oils from Fennel fruit(Foeniculum vulgare Mill), Olibanum resin(Boswellia carteii Birew) and Needl Juniperus stem(Juniperus rigida Sieb.) were extracted by a supercritical fluid extraction system(SFE) and biological activity of each essential oils were observed. SFE technique was applied for the isolation and purification of nonpolar biologically active essential oils from each samples. The quantitative analysis of essential oils was carried out by gas chromatography-mass spectrometer(GC/MS). About 60% of the growth of AGS and A549 cells were inhibited by adding 1.0g/l of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell(HEL299) was scored as $15{\sim}18%$ for the crude essential oils and 12% for control, respectively. It meant that the essential oils were more effective than the control in anti-mutagenecity tested by CHO V79 cells. The effect of the essential oils on the growth of nerve cells, PC12 was observed as follows: The viable cell density was about two times higher than control.

전통적인 향기치료제로 쓰여 온 회향, 유향 그리고 노간주를 사용하여 최근 신기술로 평가되고 있는 초임계 유체추출법을 통해 생체 조절기능을 지닌 기능성 정유 성분을 추출하였다. 기존의 SDE 추출법과 비교하여 추출수율 측면에서 비교적 높은 결과를 얻어내어 정유 추출시 장차 SDE 추출법을 대체할 신기술로 평가되었다. 추출용매를 사용해야 하는 SDE 추출법과는 달리 무색, 무취인 $CO_2$를 이용하여 정유 성분을 분리하는 초임계 추출법은 인체에 무해한 정유 성분을 얻을 수 있다는 것이 가장 큰 장점이라 사료되며, 분리된 정유 물질에 대해 성분 분석 결과 대부분이 휘발성 알코올 계열에 물질임을 확인할 수 있었다. 정유 성분들에 대한 생리활성 실험결과 항암, 항 돌연변이 그리고 신경활성이 아주 높은 것으로 나타나 aroma-theraphy로서의 가능성을 제시하였다고 사료된다. 나아가 이 정유성분에 대한 in vivo 실험을 통해 기초 의약품으로서 연구가 수행되어진다면 전통적인 치료제로서 사용된 천연물에 대한 현대적 가치부여가 가능하다고 생각된다.

Keywords

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