Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Extraction and Biological Activities of Essential Oil from Thuja occidental Leaves

서양측백나무 잎으로부터 식물정유 추출 및 생리활성

  • 서원택 (진주산업대학교 식품가공학과) ;
  • 양재경 (경상대학교 산림과학부) ;
  • 강병국 (경상대학교 산림과학부) ;
  • 박우진 (경상대학교 산림과학부) ;
  • 홍성철 (경상대학교 산림과학부) ;
  • 강영민 (경상대학교 산림과학부) ;
  • 정희영 (경상대학교 산림과학부) ;
  • 김용덕 (경상대학교 산림과학부) ;
  • 강승미 (경상대학교 산림과학부) ;
  • 김선원 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 농업생명과학연구원)
  • Published : 2003.12.31
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Abstract

Essential oil of Thuja occidental leaves was extracted by steam distillation method, and chemical composition of essential oil was analyzed by GC-MS. The essential oil was tested to antimicrorbiaI activity against 28 test microorganisms and antioxident assay through DPPH free radical scaveging test and insecticidal activity by mosquito insecticide assay. The major constituents in the essential oil of T. occidental were monoterpene as d-isothujone, ${\alpha}-thujone$, camphor, L-fenchone, and hornyl acetate. The essential oil have broad antimicrobiaI activity, which showed strong antimicrobiaI activity for Streptococcus pyogenes as bacteria, Alternaria alternata, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Streptococcus mutans as fungi. The essential oil also showed DPPH free radical scavenging ability at concentration of $36\;{\mu}{\ell}/m{\ell}$. In spraying and fumigation tests, the essential oil had strong insecticidal activity against mosquito.

서양측백나무의 잎으로부터 정유를 steam distillation법으로 추출하고, GC-MS로 정유 조성성분을 분석하였으며, 28종의 미생물종을 대상으로 항균활성을, DPPH법으로 항산화 활성을, 집모기에 대한 살충효과를 검정하였다. 서양측백나무 정유 주성분은 monoterpene류였으며, d-isothujone, l-fenchone, ${\alpha}-thujone$, camphor, terpin-4-ol 순으로 나타났다. 정유의 박테리아에 대한 항균력은 박테리아 중 Streptococcus pyogenes에서, 그리고 효모 및 곰팡이 중 Candida albicans에서 가장 높게 나타났다. 특히 서양측백나무 정유는 그램 양성균의 박테리아에 대해 가장 장한 항균력을 보였다. 정유는 DPPH radical 소거능을 보유하고 있었으며, $EC_{50}$ 값은 $36\;{\mu}{\ell}/m{\ell}$이었다. 집모기에 대한 살충 효과는 분무, 훈증, 자연증발 등 처리방법에 따라 다르게 나타났으며, 그 중 분무처리가 가장 효과적인 것으로 나타났다. 이상의 결과는 환경 친화적인 항균 및 살충소재로 이용될 수 있을 것이다.

Keywords

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