Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Standardization of Pancreatin

판크레아틴의 규격 표준화 연구

  • Published : 2003.12.20
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Abstract

Pancreatin is a enzyme mixture breaking down carbohydrates, proteins and lipids. Most pancreatin used in Korea is imported from foreign countries. However, guideline of each country for pancreatin produced from each country is different. Therefore, guideline for pancreatin imported from several countries, such as Europe, Japan and America, it is standardized to control its quality. Assay of enzyme activity for pancreatin in KP is similar to tat in JP, but it is significantly different from those in FP ad in USP. We measured pancreatin digestive activities of 17 commercial products. Activity assay of digestive enzymes, starch- and lipid-digestive enzymes, for pancreatin by KP method (including JP) was difficult compared to those by FIP ad USP methods. Particularly, activity assays of starch- and lipid-digestive enzymes by KP method were mistakable, ad varied in diluted samples than those by FIP. However, activity assay of protein-digestive enzyme by KP method was similar to that by FIP. Starch-digestive enzyme activities of 17 commercial pancreatins by KP method were lower 0.079-fold compared to those by FIP method. Their protein-digestive enzyme activities by KP method were higher 75.7-fold than those by FIP method. Their lipid-digestive enzyme activities by KP method were lower 0.234-fold compared to those by FIP method.

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References

  1. 대한약전 제 8 개정, 판크레아틴, 1016-1017 (2002)
  2. European Pharmacopeia (EP4), Pancreatin Powder, 1701-1703 (2002)
  3. U.S. Pharmacopeia (USP26), Pancreatin, 1389-1391 (2003)
  4. 일본약국방 제 14 개정 해설서, 판크레아틴, D-929-931 (2001)
  5. T. Yamato, I. Miyara, S. Yamato, K. Fujita and K. Mizokami, $\alpha\alpha$-amylase of Rhyzopus niveus: Its isolation and some enzymetic properties, Denpun Kagaku, 37(3), 129-136 (1990).
  6. C. Yook and J.F. Robyt, Reactions of alpha amylases with starch granules in aqueous suspension giving products in solution and in a minimum amount of water giving products inside the granule, Carbohydr. Res., 337, 1113-1117 (2002) https://doi.org/10.1016/S0008-6215(02)00107-6
  7. C.M.L. Franco, S.J.R. Preto and C.F. Ciacco, Factors that affect the enzymatic degradation of natural starch granuleseffect of the size of the granules, Starch/Starke, 44, 422-426 (1992) https://doi.org/10.1002/star.19920441106
  8. S.Y. Lee and J.S. Lee, Detection and determination of lipase activity, Journal of Microbiology and biotechnology, 4(2), 85-94 (1994)
  9. G. Bengtsson and T. Olivecrona, On the pH dependency of lipoprotein lipase actvity. Biochem. Biocphys. Acta., 712, 196 (1982) https://doi.org/10.1016/0005-2760(82)90102-3
  10. Z. Saito, Application of the phenol-red method for investigation on the lipolysis of raw milk, Jap. J. Zootech. Sci., 50, 710 (1979)