Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of an Antifungal Compound from Aerial Parts of Platycarya strobilacea

굴피나무(Platycarya strobilacea) 지상부로부터 항진균성 활성물질 분리

  • Chae, Sang-Gi (Department of Plant Resources, Sangju National University) ;
  • Kim, Jin-Ho (Department of Plant Resources, Sangju National University) ;
  • Kang, Sang-Jae (Department of Horticulture, Sangju National University) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyunghee University) ;
  • Han, Jae-Taek (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyunghee University) ;
  • Choi, Yong-Hwa (Department of Plant Resources, Sangju National University)
  • 채상기 (상주대학교 식물자원학과) ;
  • 김진호 (상주대학교 식물자원학과) ;
  • 강상재 (상주대학교 원예학과) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한재택 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 최용화 (상주대학교 식물자원학과)
  • Published : 2003.08.30
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Abstract

Methanol extract obtained from aerial parts of Platycarya strobilacea was successively fractionated with n-hexane, ethylacetate, n-butanol, and water. From ethylacetate fraction, an active compound was isolated through repeated silica gel column chromatography and was identified as 5-hydroxy-2-methoxy-1,4-naphthoquinone by MS and NMR analyses. The compound showed in vivo 76% antifungal activity at $100\;{\mu}g/ml$ against tomato late blight disease.

굴피나무 지상부의 메탄을 추출물을 n-hexane, ethylacetate, n-butanol, $H_2O$으로 순차적으로 용매분획하였다. Ethylacetate 분획으로부터 silica gel chromatography를 반복하여 활성물질을 분리 정제하였다. 화합물은 NMR과 MS의 기기분석 결과 5-hydroxy-2-methoxy-1,4-naphthoquinone로 구조결정 되었다. 이 화합물은 in vivo 실험결과 토마토역병에 대하여 $100\;{\mu}g/ml$에서 76%의 방제효과를 나타내었다.

Keywords

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