Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Monitoring of Genetically Modified Soybean and Processed Foods in Korean Market using PCR

PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

  • Kim, Myo-Young (College of Life Sciences, Kyung Hee University) ;
  • Kim, Jae-Hwan (College of Life Sciences, Kyung Hee University) ;
  • Kim, Hyun-Joong (College of Life Sciences, Kyung Hee University) ;
  • Park, Sun-Hee (Divisions of Food Microbiology, Korea Food and Drug Administration) ;
  • Woo, Geon-Jo (Divisions of Food Microbiology, Korea Food and Drug Administration) ;
  • Kim, Hae-Yeong (College of Life Sciences, Kyung Hee University)
  • 김묘영 (경희대학교 생명과학대학) ;
  • 김재환 (경희대학교 생명과학대학) ;
  • 김현중 (경희대학교 생명과학대학) ;
  • 박선희 (식품의약품안전청 식품미생물과) ;
  • 우건조 (식품의약품안전청 식품미생물과) ;
  • 김해영 (경희대학교 생명과학대학)
  • Published : 2003.11.30
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Abstract

A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 콩 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 epsps가삽입된 CMS가 검출되었으나, 표시제 이후에는 검출되지 않았다.

Keywords

References

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