Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation of Polyphenol from Green Tea by HPLC and Its Physiological Activities

HPLC에 의한 녹차의 polyphenol 화합물의 분리 및 polyphenol의 생리활성

  • Woo, Hee-Seob (School of Food Science, Dongju College) ;
  • Choi, Hee-Jin (Department of Food Science and Technology, Yeungnam University) ;
  • Han, Ho-Suk (Department of Food Science and Technology, Yeungnam University) ;
  • Park, Jung-Hye (Department of Food Science and Technology, Yeungnam University) ;
  • Son, Jun-Ho (Department of Food Science and Technology, Yeungnam University) ;
  • An, Bong-Jeun (Faculty of Life Resource and Engineering, Kyungsan University) ;
  • Son, Gyu-Mok (Division of Food Science, Changwon Junior College) ;
  • Choi, Cheong (Department of Food Science and Technology, Yeungnam University)
  • 우희섭 (동주대학 식품과학계열) ;
  • 최희진 (영남대학교 생물산업공학부) ;
  • 한호석 (영남대학교 생물산업공학부) ;
  • 박정혜 (영남대학교 생물산업공학부) ;
  • 손준호 (영남대학교 생물산업공학부) ;
  • 안봉전 (경산대학교 생명자원공학부) ;
  • 손규목 (창원전문대학 식품영양과) ;
  • 최청 (영남대학교 생물산업공학부)
  • Published : 2003.12.01
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Abstract

Polyphenols were isolated from Korean green tea using Sephadex LH-20 and HPLC. The isolated polyphenols were procyanidin B-4, procyanidin B-2-3,3'-digallate, prodelphinidin C-2-3,3'-di-O-digallate, (+)-catechin-3-O-rhamnose, procyandin B-5, procyanidin B-7-3-0-gallate, gallate, epiafzelechin-$(4{\beta}{\rightarrow}8)$-epiafzelechin, procyanidin B-3-3-O-rhamnose, afzelechin-$(4{\alpha}{\rightarrow}8)$-catechin, prodelphinidin B-5-3,3'-di-O-digallate and (+)-taxifolin-3-O-D-xyloside. The inhibitory effects of prodelphinidin C-2-3,3'-di-O-gallate and procyanidin B-2-3,3'-digallate $(at\;100{\mu}M)$ on angiotensin.converting enzyme were 68.8 and 54.6%, respectively, while the inhibitory effects of prodelphinidin C-2-3,3'-di-O-gallated and procyanidin B-2-3,3'-digallate $(at\;100{\mu}m)$ on xanthine oxidase were 54.5 and 38.2%, respectively. Lastly, the inhibitory activities of prodelphinidin C-2-3,3'-di-O-gallate $(at\;100{\mu}m)$ on tyrosinase was 42.1%.

한국산 녹차를 70% 에탄올로 추출한 후 Sephadex LH-20과 HPLC를 사용하여 polyphenol을 분리동정하였고 분리된 각 성분들의 angiotensin converting enzyme(ACE) 및 xanthine oxidase, tyrosinase의 저해효과를 확인하였다. 녹차로부터 HPLC로 polyphenol을 분리한 결과 epiafzelechin-$(4{\beta}{\rightarrow}8)$-epiafzelechin, procyanidin B-3-3-O-rhamnose, afzelechin-$(4{\alpha}{\rightarrow}8)$-catechin, prodelphinidin B-5-3,3'-di-O-digallate, (+)-taxifolin-3-O-D-xyloside 등 11종류를 분리하였다. ACE의 저해효과는 prodelphinidin-C-2-3,3'-di-O-gallate $100{\mu}M$에서 68.8%, procyanidin B-2-3,3'-digallate가 54.6%의 저해를 나타내었고, Xanthine oxidase는 prodelphinidin C-2-3,3'-di-O-gallate가 54.5%, procyanidin B-2-3,3'-digallate가 38.2%로 높은 저해효과를 보였다. Tyrosinase의 저해효과는 $100{\mu}M$ 농도에서 prodelphinidin C-2-3,3'-di-O-gallate가 42.1%, procyanidin B-2-3,3'-digallate와 procyanidin B-7이 각각 30.7%, 20.5%의 저해를 나타내었다. 따라서 녹차 추출물로부터 분리한 polyphenol이 혈압예방, 통풍 및 미백효과의 기능성 식품 신소재로서 이용이 가능하다는 것을 확인하였다.

Keywords

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