Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Activities of Castanea Crenata Flos. Methanol Extracts

밤꽃(Castanea Crenata Flos.) 메탄올 추출물의 항산화 효과

  • Choi, Chang-Suk (Department of Food Science and Nutrition, Hoseo University) ;
  • Song, En-Sung (Department of Food Science and Nutrition, Hoseo University) ;
  • Kim, Jang-Su (Graduate School of Venture, Hoseo University, Su-Jin BioTech. Co. LTD) ;
  • Kang, Myung-Hwa (Department of Food Science and Nutrition, Hoseo University, Graduate School of Venture, Hoseo University)
  • 최창숙 (호서대학교 자연과학부) ;
  • 송은승 (호서대학교 자연과학부) ;
  • 김장수 (호서대학교 벤처전문대학원 첨단기술, (주)수진바이오텍) ;
  • 강명화 (호서대학교 자연과학부, 호서대학교 벤처전문대학원 첨단기술)
  • Published : 2003.12.01
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Abstract

The antioxidative activities of methanol extracts of chestnut flower (Castanea Crenata Flos.) were determinated in vitro using an experimental model system. Solid yield of chestnut flower extracts was 6.26% and total phenolic acid accounted for about 20% of the crude extract. The DPPH radical scavenging activity of methanol extracts prepared from chestnut flower was 17.22%. Although the DPPH radical scavenging activity of chestnut flower extracts was lower than that of other antioxidants, chestnut flower extracts showed continuous activity by time course. The SOD-like activities of methanol extracts prepared from chestnut flower were 65.10%, 95.70% in BHT, 93.29% in quercetin, and 30.30% in ascorbic acid. Chestnut flower extracts showed 51.45% inhibitory effect on peroxidation of egg yolk lecithin.

밤꽃의 항산화효과를 측정하기 위해 밤꽃의 수꽃을 수집하여 메탄올로 추출한 후 추출물의 항산화 효과를 측정하였다. 밤꽃 조추출물의 추출수율은 6.26% 였으며, 총 페놀함량은 0.199 mg/mL 인 것으로 나타났다. DPPH법으로 free radical 소거능을 측정한 결과 밤꽃 추출물이 17.22%로 기존의 항산화제에 비해서는 소거능이 다소 낮은 것으로 측정되었다. SOD 유사활성 측정은 밤꽃 추출물이 65.10%로 BHT(95.70%)와 quercetin(93.29%)에 비해 낮았지만, ascorbic acid(30.30%) 보다는 높은 활성을 나타내었다. Lecithin 산화저해율은 밤꽃 추출물이 51.45%, BHT(67.08%), quercetin(63.46%), ascorbic acid(23.60%), chlorogenic acid(48.76%), tannic acid(50.10%)로 나타났다. 이상의 연구결과 밤꽃 추출물이 DPPH free radical 소거능에서 기존 항산화제에 비해 낮은 활성을 나타냈지만, 시간경과에 따른 활성의 지속성과 50% 이상의 지질과산화 억제효과가 있어 천연항산화제로서의 가능성이 충분히 있는 것으로 추정된다.

Keywords

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