Anticariogenic Activities of Various Plant Extracts

항충치효과를 가진 식물 소재 탐색

  • Published : 2003.12.01

Abstract

As an effort to select powerful anti-cariogenic materials from natural resources, various plant extracts were examined for their anti-S. mutans and anti-glucosyltransferase (GTase) activities. The ethanol extracts of licorice bark, which was produced after water extraction of licorice, showed the most powerful anti-S. mutans as well as anti-GTase activities. When licorice bark was consecutively fractionated with n-hexane, chloroform, ethylacetate, and butanol, the chloroform fraction exhibited the strongest anti-S. mutans activites. This fraction was further fractionated into 4 fractions through a silica gel column, and according to HPLC analysis, anti-S. mutant activities seemed to come mostly from relatively hydrophobic materials.

충치발생 원인균인 Streptococcus mutans에 대한 각종 천연물질의 항균효과와 GTase 저해활성을 조사한 결과, 미숙사과, 황련, 우롱차, 감초 등에서 항충치 효과를 나타내었으며, 그 중에서 감초를 열수추출한 다음 생성된 박을 75% 에탄올로 추출하여 얻어진 감초박 추출물이 가장 탁월한 생육저해 효과가 관찰되었다. 또한 이들 소재를 중심으로 충치균이 불용성 glucan을 생산하는 GTase 활성 저하에 미치는 영향을 살펴본 결과, 녹차, 오배자, 미숙사과 polyphenol 추출물 및 감초박 추출물이 GTase의 활성을 저해시키는 효과를 보였으며 특히 항충치 효과에서와 같이 감초박을 75% 에탄올로 추출한 감초박 추출물이 가장 뛰어난 GTase 활성억제 효과를 나타내었다. 감초박을 75% 이상의 에탄올로 추출한 추출물보다 chloroform으로 추출한 추출물에서 더욱 탁월한 항충치균 효과가 관찰되었다. 감초박을 75% 에탄올로 추출한 후, 각종 유기용매로 2차 분획하였을 때, chloroform으로 추출한 분획에서 가장 강한 항충치균 활성이 나타났다. 얻어진 추출물을 silicagel column상에서 4개의 분획으로 재차 분리하였고, 첫번째 분획에서 가장 강한 항충치균 활성을 나타내는 분획을 얻었으며, 이들 분획을 HPLC로 분석했을 때 항충치균에 대한 활성은 주로 소수성이 강한 물질에 의한 것이라고 추정되어졌다.

Keywords

References

  1. Hamada, S., Ooshima, T, Torii, M., Imanishi, H., Masuda, N., Mizuno, J., Sobe, S. and Kotani, S. Dental caries induction in experimental animals by clinical strains of Streptococcus mutans isolated from Japanese children. Microbiol. Nmmunol. 22: 301314(1978)
  2. Hamada, S. and Slade, H.D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol. Rev. 44: 331-384 (1980)
  3. Hamada, S., Koga, T and Ooshima, T Virulence factors of Streptococcus mutans and dental caries prevention. J. Dent. Res. 63: 407-411 (1984) https://doi.org/10.1177/00220345840630031001
  4. Koga T., Okahashi, T, Asakawa, H. and Hamada, S. Adherence of Streptococcus mutans to tooth surface, pp. 111-120. In: Molecular Microbiology and Imunobiology of Streptococcus mutans. Hamada, S., Michalek, M., Kiyono, H., Menaker, L. and McGhee, J.R. (eds.). Elsevier Science Publishers, Amsterdam, Netherlands (1986)
  5. Degar, M.D. and Walker, G.J. Metabolism of the polysaccharides of human plaque. Caries Res. 9: 21-28 (1975) https://doi.org/10.1159/000260139
  6. Gibbon, R.J. and Van-Houte, J. On the formation of dental plaque. J. Periodontol. 44: 347-360 (1973) https://doi.org/10.1902/jop.1973.44.6.347
  7. Jang, G.H., Ahn, B.Y, Oh, S.H., Choi, D.S. and Kwon, Y.J. Anticariogenic effects of Coptis chinensis franch extract. Korean J. Food Sci. Technol. 32: 1396-1402 (2000)
  8. You, Y.S., Park, K.M. and Kim, Y.B. Antimicrobial activity of some medical herbs and spices against Streptococcus mutans. Korean J. Appl. Microbial. Biotechnol. 21: 187-191 (1993)
  9. Cho, Y.J. Isolation of 3-galloylprocyanidin B_{3}, a glucosyltransferase inhibition from the Korean green tea leaves. Agri. Chem. Biotechnol. 43: 273-276 (2000)
  10. Nakahara, K., Kawabata, H.O., Ogura, K., Tanaka, T, Ooshima, T and Hamada, S. Inhibitory effect of oolong tea polyphenols on glucosyltransferases of mutans Streptococci. Appl. Environmental Microbiol. 4: 968-974 (1993)
  11. Ooshima, T., Izumitani, A., Minami, T., Fujiwara, Y., Nakajima, Y. and Hamada, S. Trehalulose does not induce dental caries in rats infected with mutans streptococci. Caries Res. 25: 277-282 (1991) https://doi.org/10.1159/000261376
  12. Ooshima, T., Izumitani, A., Minami, T., Yoshida, S. and Hamada, S. Noncariogenicity of maltitol in specific pathogen-free rats infected with mutans streptococci. Caries Res. 26: 33-37 (1992) https://doi.org/10.1159/000261424
  13. Miyoshi, M., Imoto, T. and Kasagi, T. Antieurodontic effect of various fraction extracted from the leaves of gymnema sylvestre. J. Yonago Med. Ass. 38: 127-137 (1987)
  14. Otake, S., Makimura, M., Kuroki, T., Nishihara, Y. and Hirasawa, M. Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Res. 25: 438-443 (1991) https://doi.org/10.1159/000261407
  15. Anon. GRAS status of licorice (Glycyrrhiza), ammoniated glycyrrhizin and mono ammonium glycyrrhizinate. Federal Register. 48: 54983-54990 (1983)
  16. Fenwick, G.R. Liquorice, Glycyrrhiza glabra L.-composition, uses and analysis. Food Chem. 38: 119-143 (1990) https://doi.org/10.1016/0308-8146(90)90159-2
  17. Ueno, R., Matsuda, T., Tago, K., Kunimi, Y. and Kanayama, T. Antimicrobial agents with solubilizers for food preservation. Japanese Patent 60,233,015, 131-137 (1985)
  18. Maruzen Chemical Co. Ltd. Food antioxidants from liquorice extracts and gallic acid. Japanese Patent 60, 44, 580, 625-628 (1985)
  19. Lutomski, J. Chemie und therapeutische verwendung von sussholz (Glycyrrhiza glabra L.). Pharm. Zeit 12: 49-54 (1983)
  20. Saitoh, T., Kinoshita, T. and Shibata, S. New isoflavan and flavanone from liquorice root. Chem. Pharm. Bull. 24. 752-755 (1976) https://doi.org/10.1248/cpb.24.752
  21. Saitoh, T., Kinoshita, T. and Shibata, S. Flavonols of liquorice root. Chem. Pharm. Bull. 24. 1242-1245 (1976) https://doi.org/10.1248/cpb.24.1242