The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Ramaria botrytis Methanol Extract on Hepatotoxicity in Benzo(α) Pyrene-treated Mice and Expression of Cytochrome P-450 1A1 Isozyme

벤조피렌 유발 마우스에서 싸리버섯 메탄올 추출물의 간 독성 억제효과 및 사이토크롬 P-450 1A1 Isozyme의 발현에 미치는 영향

  • Kim, Hyun-Jeong (Traditional Microorganism Resources Center, Keimyung University) ;
  • Lee, In-Seon (Traditional Microorganism Resources Center, Keimyung University) ;
  • Bae, Jun-Tae (Department of Food and Nutrition, Yeungnam University) ;
  • Kim, Ok-Mi (Department of Hotel Culinary Arts, Taekyeung College) ;
  • Park, Sun-Hee (Department of Food and Nutrition, Yeungnam University) ;
  • Chang, Jong-Sun (Department of Food and Nutrition, Yeungnam University) ;
  • Park, Jun-Hong (Department of Food and Nutrition, Yeungnam University) ;
  • Lee, Kap-Rang (Department of Food and Nutrition, Yeungnam University)
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 이인선 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 배준태 (영남대학교 식품영양학과) ;
  • 김옥미 (대경대학 호텔조리계열) ;
  • 박선희 (영남대학교 식품영양학과) ;
  • 장종선 (영남대학교 식품영양학과) ;
  • 박준홍 (영남대학교 식품영양학과) ;
  • 이갑랑 (영남대학교 식품영양학과)
  • Published : 2003.04.30
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Abstract

This study was conducted to investigate effects of Ramaria botrytis methanol extract on liver damage in benzo$({\alpha})$pyrene(B$({\alpha})$P)-treated mice. The activities of serum amminotransferase, cytochrome P-450, aminopyrine N-demethylase, aniline hydroxylase and hepatic content of lipid peroxide after B$({\alpha})$P-treatment were increased than control, but those levels were significantly decreased by the treatment of Ramaria botrytis methanol extract. Whereas, the hepatic glutathione content and activities of glutathionie S-transferase and r-glutamylcysteine syntherase were increased by the treatment of Ramaria botrytis methanol extract. In addition, cytochrome P-450 1A1 izozyme protein level, remarkably increased by B$({\alpha})$P-treatment was decreased by the treatment with methanol extract of Ramaria botrytis. These results suggest that the protective effect of methanol extract of Ramaria botrytis on liver injury in B$({\alpha})$P-treated mice may be due to reduction of oxygen free radical.

싸리버섯 메탄올 추출물이 간독성 물질인 B$({\alpha})$P을 투여한 마우스에서 간 손상 억제 효과 및 cytochrome P-450 1A1 발현에 미치는 영향을 살펴보았다. B$({\alpha})$P 투여로 인한 혈청 ALT와 AST의 활성, 간 조직증의 lipid peroxide 함량, cytochrome P-450 함량, AD 및 AH 활성이 유의적으로 증가하였으며 싸리버섯 메탄올 추출물의 투여시 이들 활성 및 함량이 유의적으로 감소하였다. 반면, 간 조직중의 GSH 함량, GST, r-glutamylcysteine synthetase의 활성은 B$({\alpha})$P만 투여한 군에 비해 싸리버섯 메탄올 추출물을 투여시 증가하였다. 또한 immuno blotting 결과로부터 B$({\alpha})$P 투여에 의해 현저히 증가되었던 cytochrome P-450 1A1 isozyme 단백질 함량이 싸리버섯 메탄올 추출물의 투여로 감소됨을 확인하였다. 이와 같은 결과로부터 싸리버섯 메탄올 추출물은 B$({\alpha})$P에 의한 간 손상에 대한 보호 효과가 있는 것으로 사료된다.

Keywords

References

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