The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Antioxidant Effect, Cell Viability and the Effect to the Gene Expression Using, cDNA Microarray Assay of Jengjengamiygin-tang

正傳加味理陳湯의 항산화효과, 세포활성 및 유전자 발현에 미치는 영향

Lee Kwang-Soo;Kim, Bong-Seok;Kwak Min-Ah;Byun Joon-Seok;Seo Jung-Chul;Han, Sang-Won
이광수;김봉석;곽민아;변준석;서정철;한상원

  • Published : 20030000

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Abstract

Objectives : This study was undertaken to determine the effect of Jengjengamiygin-tang on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells. Methods : Antioxidant effect of Jengiengamiygin-tang was compared with. that of vitamin C on DPPH radical, respectively. Cell viability was determiend by MTT assay. Finally, gene expression was evaluated by cDNA microarray assay. Results : 1. Scavenging effect of Jengjengamiygin-tang on DPPH treated cells were demonstrated by differential antioxidant effect in Jengiengamiygin-tang-treated group at the dose of Img/${\ell}$. 2. Cell viability was increased in Jengiengamiygin-tang-treated group at the dose of 100 and l,${\mu}m/m{\ell}$. 3. Among 8000 unigenes, 16 genes were upregulated while 389 genes were downregulated by Jengjengamiygin-tang treatment on human stomach cancer cell line. Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.

Keywords

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