The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Salviae Multiorrhizaei Radix on Cultured Mouse Myocardial Cells Damaged by Hydrogen Peroxide

단삼이 활성산소로 손상된 배양 심근세포에 미치는 영향

Lee, Jong-Hwa;Lee Byung-Chan;Park Seung-Taeck;Lee Jung-Hun;Lee Kang-Chang;Seo, Bu-Il;Song Ho-Joon
이종화;이병찬;박승택;이정헌;이강창;서부일;송호준

  • Published : 20030000

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Abstract

Objectives : To clarify the cardotoxicity and the protective effect of Salviae Multiorrhizaei Radix(SMR) on oxygen free radicals in cultured myocardial cells of neonatal mouse. Methods : Cardiotoxicity was measured by MTT assay after myocardial cells were grown for 5 hours in the media containing 1-30 $\mu$M concentrations of $H_2O_2$. In addition. the protective effect of Salviae Multiorrhizaei Radix(SMR) was determined in these cultures. Results : Cell viability was significantly decreased in dose- and time-dependent manners after hours. In the protective effect, SMR exposure of 15 $\mu$M $H_2O_2$ to cultured rat myocardial cells for 5 prevented the $H_2O_2$-induced cytotoxicity in these cultures. Conclusion : From the above results, it suggests that $H_2O_2$ has toxic effect on cultured mouse myocardial cells and SMR blocked the cardiotoxicity induced by $H_2O_2$.

Keywords

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