Abstract
Objective : Retention enema therapy acts on the limited part directly and it can decrease side effect and maximize the effect of medication. Retention enema therapy have effect on incurable diseases so it is used frequently in clinic. This experiment was made for comparative study of anti-inflammatory effects of between oral and rectal medication. Method : The female ICR(20-30g) mice were used, the temperature was controlled within $22{\pm}2{\circ}C$ and water and food was not limited. The environment was manipulated to simulate 12 hours of day and 12 hours of night. After lipopolysaccharide was injected into peritoneum we confirmed on-set of inflammation. We started to dose Gangyeong- Tang divided into pre-treatment group(before LPS injection medicated Gangyeong-Tang group) and treatment group(after LPS injection medicated Gangyeong-Tang group) in oral and rectal, after mesured the WBC Count, TNF -${\alpha}$ concentration of blood which was collected from the Retro-orbital Plexus. Results : 1. The number of WBC in the pre-treatment group was decreased significantly(p〈0.001) as we medicated Gangyeong-Tang in oral and rectal, espescially the rectal medication group showed dose-dependent decrease. 2. The number of WBC in the treatment group was decreased significantly(p〈0.001) as we medicated Gangyeong-Tang in oral and rectal, espescially the rectal medication group showed dose-dependent decrease. 3. The concentration of TNF -${\alpha}$ in the pre-treatment group was decreased significantly as we medicated Gangyeong- Tang in oral and rectal, espescially the rectal medication group showed dose-dependent decrease. 4. The concentration of TNF-${\alpha}$ in the treatment group was decreased significantly as we medicated Gangyeong-Tang in oral only. As a result of this experiment, we demonstrated anti-inflammatory effect of Gangyeong- Tang between oral and rectal medication, availability of the retention enema therapy and anti-inflammatory effects of Gangyeong-Tang