The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지

A Study of Yukmijihwangtang-Jahage extract on Regulation of Bone Resorption

Lee, Dong-Nyung;Kim, Hyung-Jun;Kim, Dong-Il;Lee Tae Kyun
이동녕;김형준;김동일;이태균

  • Published : 20030000

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Abstract

전통 한약제제인 육미지황탕가자하거(Yukmijihwangtang-Jahage; YJ)는 7가지 약물인 Rehmannia glutinosa LIBOSCH.(熟地黃 : 16 g), Dioscorea japonica THUNB.(山藥 : 8 g), Cornus officinalis SIEB. Et. ZUCC.(山茱萸 : 8 g), poria cocos WOLF.(백복령 : 6 g), Paeonia suffruticosa ANDR.(牡丹皮 : 6 g), Alisma plantago-aquatica var. orientale SAMUELS(澤瀉 : 6 g)} and Placenta hominis(紫河車 : 20 g)로 구성되어 있으며, 본 약제는 면역학적으로 골대사활성이 있는 것으로 알려져 있다. 이에 본 연구에서는 YJ의 항골다공증활성을 사이토카인 활성, prostaglandin 생합성과 관련하여 분자세포생물학적으로 검증하였다. 즉 cyclooxygenase-2(COX-2)와 Tyrosine Kinase가 골아세포에서 IL-1${\beta}$, TNF-${\alpha}$, IL-6에 의해 유발되는 $PGE_2$생합성에 직접관여하는 사실을 밝혔다. IL-1${\beta}$와 IL-6 그리고 약간의 활성만 보이는 TNF-${\alpha}$가 COX-2 m-RNA을 합성을 촉진하였으나 TGF-${\beta}$와 YJ 추출물 및 TGF-${\beta}$+YJ 추출물의 혼합처리로 cytokine 유발 COX-2 mRNA 량 및 PGE2 생성량을 현저히 줄였다. 또한 YJ 추출물로 처리한 경우 Tyrosine Kinase가 peptide 기질의 인산화 반응을 억제함으로서 IN vivo 및 In vitro에서 골재흡수를 억제하엿다. YJ 추출물이 PTH 처리한 골재흡수억제 $IC_{50}$ 용량은 16 ${\mu}g/ml$ 이었다. 이러한 결과들로부터 YJ 추출물은 골대사과정중 골재흡수를 억제하는데 효과적임을 밝히게 되었으며, YJ에 의한 골다공증의 억제기전은 골재흡수관련 단백질들의 전사조절에 있음을 최초로 규명하게 되었다.

Keywords

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