The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지

Experimental Studies on Thymus Cells Differentiation by the Extracts of Stigma Croci

藏紅花 抽出物이 胸腺 細胞 分化에 미치는 影響

Seo Young-Bae;Kil Gi-Jung;Lee Young-Cheol;Kim Dong-Hee
서영배;길기정;이영철;김동희

  • Published : 20030000

⟨ Previous Next ⟩

Abstract

Objective : This study was carried out for the purpose of knowing the effect from thymus T cell differentiation of the C57BL/6 mice by the extraction from a ESC. Methods : The second costimulatory signal determines the activation threshold and the functional out come of the antigen-. specific activation. The activation threshold is mtdulated by costimulatory signals, and their dysregulation may play a critical role in the activation of autorextive T cells. CD80/CI)%-CrK 8/CTL.k4 is the most important costimulatory molecules. Th1 cells prduce IIW v and II-2 and play a n impxtant rolt, in allograft rejection, whereas Th2 cells stcrete LL-4, Jl-5, lL6. 11, 10, and IL- 13 and are associated with the prolongation of graft survival and tolcrrince induction. IIowever. this Thl,/Th2 p:uxiigm is more complex. CD4CD25+ T cells were shown to control autoreactive T cells, and CDU mediates leukocyte activation ii nd CD69 acts as a costimulatory moltcule for T cell activation and proliferation. So we investigated the effects of ESC on these cytokines and cell surface antigens(CD25, CD28, CD44. CD69. CD80) expressed by T cells following activation. Results : 1. In order to know the effect of the cytotoxicitp from extraction of EC, we had to examine the safe density of that on lung fibroblast cells of the mouse(m1,I;C;s). ESC did not show cytotoxicity against rnLFCs. 2. In the proliferous effect of thymus T cells, water extracts of ESC playtd an important. role on the proliferating thymus T cells compared with control group at high density. 3. ESC promoted the CD80(B7-1) and T h l cytokines especially IFN V, IL-2, IL-4 gene exprtbssion, but not CTLA-4, T h F a , CrY28 gene expression. 4. ESC up- regulates CD44'CD69'CD'L5', CD4'CD25', CD8'CD25', CLB'CDXO' expression but not CD3'CD2 8' by anti--CD3-stimulated T cells. 5. Binding activity of transcription factors(NF-KB NFAT, AP-1) was increased by ESC. We thought that ESC also could regulate the expression of a variety of inducible genes by increasing the binding activity of transcription factors(NF-KB, NFAT, A1'- 1 ). Conclusions : Above results indicates that ESC play an important role in the activation of autoreactive T cells by promoting The cytohne(IFN-$\gamma$, II-2) and cftlcreasiny C T A-4 , CD28 and Th2 cytokines IL-4 gene expression, by up-regulating cell surface molecule(CD80, CD35, 1144). Binding activity of transcription factor ~(NF-KB. NFAT, AP-1) was increased by ESC. We thought that ESC also could regulate the expression of a variety of inducible genes by increasing the binding activity of transcription factordNF-KB, NFAT, AP-1). Therefore further deep studies shoud be accomplished about its mechanisms.

Keywords

References

  1. 圖解鄕藥(生藥)大事典(植物編) 鄭普燮;辛民敎
  2. 中華本草(八卷) v.8 國家中醫藥管理局<中華本草>編委會
  3. 飮膳正要 忽思慧(撰)
  4. 本草綱目(上冊) 李時珍
  5. 中藥大事典(下) 中藥大辭典編纂委員會(編)
  6. J. Ethnopharmacol v.31 no.1 Modulatory effects of Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in mice Nair SC;Salomi MJ;Panikkar B;Panikkar KR.
  7. Nutr Cancer v.35 no.2 Effects of long-term treatment of colon adenocarcinoma with crocin, a carotenoid from saffron(Crocus sativus L.): an experimental study in the rat Garcia-Glmo DC;Riese HH;ESClibano J;Ontanon J;Fernandez JA;Atienzar M;Garcia-Olmo D.
  8. Cancer Lett v.100 no.1-2 Cracin, safranal and picrocrocin from saffron(Crocus sativus L.) inhibit the growth of human cancer cells in vitro Escribano J;Alonso GL; Coca-Prados M;Fernandez JA
  9. Cancer Biother v.10 no.4 Saffron chemoprevention In biology and medicine: a review Nair SC;Kurumboor SK;Hasegawa JH.
  10. Clinical Tumor Immunology T-cell rosettes in human cancer Wybran J.;Fudenberg HH.;Wybran J;Staquet M(eds)
  11. J Clin Invest. v.52 Thymus derived rosette forming cells in various disease states. Cancer, lymphoma, bacterial and viral infections and other diseases Wybran J;Fudenberg HH.
  12. J. Natl. Cancer Inst. v.82 New Colorimetric Cytotoxicity Assay for Anticancer Drug Screening Philip Skehan;Ritsa Storeng;Dominic Scudiero;Anne Monks;James McMahon(et aI.)
  13. 現代中醫藥應用與硏究大系(中藥) 施杞(總主編)
  14. J Immunol v.148 SGeneration, propagation and targeting of human CD4+ helper/killer T cells induced by anti-CD3 monoclonal antibody plus recombinent IL-2 Nishimura T;Nakamura Y;Takeuchi Y;Tokuda Y;Iwasawa M;Kawasaki A;Okumura K;Habu
  15. The Journal of Immunology v.166 Acquisition of CD80(B7-l) by T Cells Helen Sabzevari;Judy Kantor;Adnan Jaigirdar;Yutaka Tagaya;Mayumi Naramura;James W. Hodge;John Bemon;Jeffrey Schlom
  16. CELLUAR MOLECULAR IMMUNOLOGY 세포$\cdot$분자 면역학 Abul K. Abbas.;김광역(외옮김)
  17. J Exp Med. v.182 no.2 CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation Krummel MF;Allison JP.
  18. Mol Cell Biol v.14 no.5 The cytoplasmic domain of CD28 is both necessary and sufficient for costimulation of interJeukin-2 secretion and association with phosphatidyJinositol 3'-kinase Stein PH;Fraser JD;Weiss A.
  19. The Journal of Immunology v.164 IL-2 Activation of NK Cells: Involvement of MKKl/2/ERK Bul Not p38 Kinase Pathway Tse-Kuan Yu;Eva G. Caudell;Christine Smid;Elizabeth A. Grimm.
  20. J. Immunol. v.162 lL-2 induces STAT4 activation in primary NK cells and NK cell lines, but not in T cells Wang, K. S.;J. Ritz;D. A. Frank.
  21. Semin Immunol. v.7 Natural killer cells wear different hats: effector cells of innate resistance and regulatory cells of adaptive immunity and of hematopoiesis Trinchieri, G.
  22. International Immunology v.14 no.2 Natural CD4CD25+ regulatory T cells control the burst of superantigen-induced cytokine production: the role of IL-10 Christiane Pontoux;Alice Banz;Martine Papiernik
  23. Immunity v.12 no.4 B7/CD28 costimulation is essential for the horreostasis of the CD4'CD25' immunoregulatory T cells that control autoimmune diabetes Salomon B.;Lenschow D.J.;Rhee L.;Ashourian N.;Singh B.;Sharpe A;Bluestone J.A.
  24. TRENDS In Molecular Medicine v.7 no.5 Cuff : A crucial role for CD44 In inflammation Ellen Pure;Carolyn A.
  25. Journal of Experimental Medicine v.164 Human T cell activation. III. Rapid induction of a phosphorylated 28 kD/32 kD disulfide-linked early activation antigen(EA-1) by 12-o-tetraconoyl phorbol-13-acetate, mitogens, and antigens Hara T;Jung LKL;Bjomdahl JM;Fu SM.
  26. Journal of Immunology v.143 T cell activation vIa Leu 23(CD69) Testi R;Phillips JH;Lanier LL.
  27. Stem Cells v.12 no.5 The activation antigen CD69 Ziegler SF;Ramsdell F;Alderson MR.
  28. Immunity v.6 Induction of peripheral T cell tolerance in vivo requires CTLA-4 engagement Perez, V. L.;L. Van Parijs;A. Biuckians, X. X. Zheng;T. B. Strom;A. K. Abbas.
  29. J. Immunol. v.160 CTLA-4 regulates tolerance induction and T cell differentiation In vivo Walunas, T. L.;J. A. Bluestone
  30. Curr Med Chem v.9 no.2 Inhibitors of AP-1 and NF-kappa B mediated transcriptional activation: therapeutic potential in autoimmune diseases and structural diversity Palanki MS.
  31. Cell Immunol v.196 no.1 Interleukin-18 induces interferon-gamma production through NF-kappaB and NFAT activation in murine T helper type 1 cells Tsuji-Takayama K;Aizawa Y;Okamoto I;Kojima H;Koide K;Takeuchi M;Ikegami H;Ohta T;Kurimoto M.
  32. Annu. Rev. Immunol. v.17 TRANSCRIPTIONAL REGULATION OF TLYMPHOCYTE DEVELOPMENT AND FUNCTION Chay T. Kuo;Jeffrey M. Leiden
  33. Biochimica et Biophysica Acta v.1498 Review The role of NF-AT transcription factors in T cell activation and differentiation Edgar Serfling;Friederike Berberich-Siebelt;Sergei Chuvpilo;Eriks Jankevics;Stefan Klein-Hessling;Thomas Twardzik;Andris Avots