Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Excessive Dietary Conjugated Linoleic Acid Affects Hepatic Lipid Content and Muscular Fatty Acid Composition in Young Chicks

  • An, B.K. (Animal Resources Research Center, College of Animal Husbandry, Konkuk University) ;
  • Shinn, K.H. (Animal Resources Research Center, College of Animal Husbandry, Konkuk University) ;
  • Kobayashi, Y. (Division of Bioresources and Bioproduction, Graduate School of Agriculture, Hokkaido University) ;
  • Tanaka, K. (Division of Bioresources and Bioproduction, Graduate School of Agriculture, Hokkaido University) ;
  • Kang, C.W. (Animal Resources Research Center, College of Animal Husbandry, Konkuk University)
  • Received : 2002.12.27
  • Accepted : 2003.04.08
  • Published : 2003.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of dietary conjugated linoleic acid (CLA) on lipid concentrations and fatty acid composition of various tissues were studied in young chicks. From 7 days of age, a total of 160 chicks were divided into 4 groups, placed into 4 pens per group (10 birds per pen) and fed one of four experimental diets containing 6% tallow (TO 6%), 4% tallow plus 2% CLA (TO 4%-CLA 2%), 2% tallow plus 4% CLA (TO 2%-CLA 4%) or 6% CLA (CLA 6%) for 3 weeks. There were no significant differences in growth performances and the relative weights of various organs, but relative liver weight of chicks fed dietary CLA at 4 and 6% levels was significantly higher (p<0.05) than that of TO 6% group. The chemical compositions of leg muscle were not affected by CLA feeding. However, hepatic total lipid of chicks fed 6% CLA diet was significantly higher (p<0.05) than those of TO 6% and TO 4%-CLA 2% groups. The concentrations of various lipid fractions in serum were not affected by CLA feeding. With the increase in dietary CLA levels, cis 9-trans 11 CLA, trans 10-cis 12 CLA and total CLA of leg muscle increased linearly. The relative proportions of C18:1 $\omega$ -9 and C20:4 $\omega$-6 fatty acids in the leg muscles of chicks fed the CLA containing diets were significantly lower (p<0.05) than those of TO 6% group. These results indicate that the levels of CLA isomers were increased linearly in dose-dependent manner after feeding of synthetic CLA source. But it was also observed that excessive amount of dietary CLA resulted in the possible adversely effects, such as increase of liver weight, hepatic lipid accumulation and serum GOT level.

Keywords

References

  1. Ahn, D. U., J. L. Sell, C. Jo, M. Chamruspollert and M. Jeffrey. 1999. Effect of dietary conjugated linoleic acid on the quality characteristics of chicken eggs during refrigerated storage. Poult. Sci. 78:922-928. https://doi.org/10.1093/ps/78.6.922
  2. An B. K., S. Ohtani and K. Tanaka. 1995. Effects of dietary vitamin B6 levels on lipid concentration and fatty acid composition in growing chicks. Asian-Aust. J. Anim. Sci. 8:627-633.
  3. AOAC. 1990. Official Methods of Analysis of the Association of Official Analytical Chemists, 15th. Ed.
  4. Aydin, R., M. W. Pariza and M. E. Cook. 2001. Olive oil prevents the adverse effects of dietary conjugated linoleic acid on chick hatchability and egg quality. J. Nutr. 131:800-806.
  5. Bauman, D. E., D. M. Barbano, D. A. Dwyer and J. M. Griinari. 2000. Technical note: production of butter with enhanced conjugated linoleic acid for use in biomedical studies with animal models. J. Dairy Sci. 83:2242-2245.
  6. Belury, M. A. and A. Kempa-Steczko. 1997. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32:199-204. https://doi.org/10.1007/s11745-997-0025-0
  7. Benito, P., G. J. Nelson, D. S. Kelley, G. Bartolini, P. C. Schmidt and V. Simon. 2001. The effect of conjugated linoleic acid on plsma lipoproteins and tissue fatty acid composition in humans. Lipids 36:229-236. https://doi.org/10.1007/s11745-001-0712-x
  8. Chamruspollert, M. and J. L. Sell. 1999. Transfer of dietary conjugated linoleic acid to egg yolks of chickens. Poult. Sci. 78:1138-1150. https://doi.org/10.1093/ps/78.8.1138
  9. Cook, M. E., C. C. Miller, Y. Park and M. Pariza. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poult. Sci. 72:1301-1305. https://doi.org/10.3382/ps.0721301
  10. Du, M. and D. U. Ahn. 2002. Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult. Sci. 81:428-433. https://doi.org/10.1093/ps/81.3.428
  11. Dugan, M. E. R., J. L. Aalhus, A. L. Schaefer and J. K. G. Kramer. 1997. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can J. Anim. Sci. 77:723-725. https://doi.org/10.4141/A97-084
  12. Duncan, D. B. 1955. Multiple range and multiple F test. Biometr 11:1-42. https://doi.org/10.2307/3001478
  13. Folch, J., M. Lee and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497-509.
  14. Ip C., S. Banni, E. Angioni, G. Carta, J. McGinley, H. J. Thompson, D. Barbano and D. Bauman. 1999. Conjugated linoleic acidenriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J. Nutr. 129:2135-2142.
  15. Jones, S., D. W. Ma, F. E. Robinson, C. J. Field and M. T. Clandinin. 2000. Isomers of conjugated linoleic acid (CLA) are incorporated into egg yolk lipids by CLA-fed laying hens. J. Nutr. 130:2002-2005.
  16. Lee, K. N., D. Kitchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25. https://doi.org/10.1016/0021-9150(94)90034-5
  17. Lee, K. N., M. W., Pariza and J. M. Ntambi. 1998. Conjugated linoleic acid decreases hepatic stearyl-CoA desaturase mRNA expression. Biochem. Biophys. Res. Com. 248:817-821. https://doi.org/10.1006/bbrc.1998.8994
  18. Lumeij, J. T. 1997. Avian Clinical Biochemistry. In: Clinical Biochemistry of Domestic Animals (Ed. J. J. Kaneko, J. W. Harvey and M. L. Bruss. 5th) Academic Press. pp. 857-883.
  19. National Research Council. 1994. Nutrients Requirements of Poultry. 9th rev. ed. National Academic Press, Washington, DC.
  20. Nicolosi, R. J., E. J. Rogers, D. Kritchevsky, J. A. Scimeca and P. J. Huth. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22:266-277.
  21. Park, Y., K. J. Albright, W. Lui, J. M. Storkson, M. E. Cook and M. W. Pariza. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858. https://doi.org/10.1007/s11745-997-0109-x
  22. SAS. 1986. SAS User's guide. Statistical Analysis System Institute. Inc., Cary, NC.
  23. Takenoyama, S., S. Kawahara, H. Murata and K. Yamauchi. 1999. Investigation of some preparation procedures of fatty acid methyl esters for capillary gas-liquid chromatographic analysis of conjugated linoleic acid in meat. Anim. Sci. J. 70:336-342.
  24. Yamasaki, M., A Ikeda, A. Hirao, Y. Tanaka, Y. Miyazaki, M. Shimada, K. Sugimachi, H. Tachibana and K. Yamada. 2001. Effect of dietary conjugated linoleic acid on the in vivo growth of rat hematoma dRLh-84. Nutr. Cancer 40:140-148. https://doi.org/10.1207/S15327914NC402_10
  25. Yamasaki, M., K. Kishihara, K. Mansho, Y. Ogino, M. Kasai, M. Sugano, H. Tachibana and K. Yamada. 2000. Dietary conjugated linoleic acid increases immunoglobulin productivity of Sprague-Dawley rat spleen lymphocytes. Biosci. Biotechnol. Biochem. 64:2159-2164. https://doi.org/10.1271/bbb.64.2159

Cited by

  1. Conjugated linoleic acid enhanced the immune function in broiler chicks vol.94, pp.05, 2005, https://doi.org/10.1079/BJN20051482
  2. Effects of conjugated linoleic acids on growth performance, serum lysozyme activity, lymphocyte proliferation,and antibody production in broiler chicks vol.59, pp.5, 2005, https://doi.org/10.1080/17450390500247808
  3. Influence of dietary fat sources and conjugated fatty acid on egg quality, yolk cholesterol, and yolk fatty acid composition of laying hens vol.47, pp.0, 2018, https://doi.org/10.1590/rbz4720170303
  4. Effects of Dietary Garlic Powder and Copper on Cholesterol Content and Quality Characteristics of Chicken Eggs vol.19, pp.4, 2003, https://doi.org/10.5713/ajas.2006.582
  5. Nutritional Values of Red Pepper Seed Oil Meal and Effects of Its Supplementation on Performances and Physiological Responses of Broiler Chicks vol.20, pp.6, 2003, https://doi.org/10.5713/ajas.2007.971
  6. Effects of Germinated and Fermented Unmarketable Soybean on Laying Performance and Egg Quality in Laying Hens vol.28, pp.5, 2008, https://doi.org/10.5851/kosfa.2008.28.5.667
  7. Conjugated Linoleic Acid as a Key Regulator of Performance, Lipid Metabolism, Development, Stress and Immune Functions, and Gene Expression in Chickens vol.22, pp.3, 2009, https://doi.org/10.5713/ajas.2009.r.04