Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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RESEARCH PAPERS : Antioxidative Properties of Baicalein, Component from Scutellaria baicalensis Georgi and Its Application to Cosmetics (I)

연구논문 : 황금(黃芩)성분, 바이칼레인의 항산화적 성질과 화장품에의 응용(제1보)

Park, Su Nam
박수남

  • Published : 20030000
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Abstract

황금(Scutellaria baicalensis Georgi)의 성분인 baicalein (5,6,7-trihydroxyflavone)을 포함하는 몇 가지 천연 항산화제들에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 보호 효과를 조사하였다. Baicalein (10 ~ 100 μM)은 농도-의존적으로 광용혈을 억제하였다. 그리고 지질 과산화 연쇄반응의 차단제인 (+)-α-tocopherol 보다도 더 효과적이었다. Baicalein의 배당체인 baicalin, 수용성 항산화제인 L-Ascorbate, 철-킬레이트제인 diethylenetriamine-N,N,N'',N'''',N''''-pentaacetic acid (DTPA)는 광용혈에 대하여 보호 효과를 나타내지 않았다. 1O2 소광제로 알려진 α-carotene-3,3''-diol (lutein)은 현저한 보호 효과를 보여주었다. 이는 rose-bengal로 증감된 광용혈에 있어서 1O2이 중요함을 가리킨다. L-Ascorbate (500 μM)는 광용혈을 촉진하였다. 하지만, ascorbate를 baicalein 용액에 첨가하면 상승적으로 보호 효과를 증가시켰다. Luminol-의존성 화학발광법을 이용하여, Fe3+-EDTA/H2O2 계에서 생성된 활성산소종(ROS)에 대한 몇 가지 천연 항산화제들의 ROS 소거활성을 측정하였다. 활성산소 소거활성(OSC50)의 크기는 DTPA 》baicalin 》L-ascorbate > (+)-α-tocopherol > baicalein 순으로 나타났다. Free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC50)의 크기는 L-ascorbate > α-tocopherol > baicalein > baicalin 순으로 나타났다. Baicalein은 rose-bengal의 광증감반응에 의하여 산화되었다. 그 산화는 ascorbate에 의해 억제되었다. 이는 광용혈에 있어서 ascorbate와 baicalein의 협동작용이 플라본을 재생시키는 ascorbate에 의한 산화된 baicalein의 환원에 기인될 수 있음을 시사한다. 이상의 결과들은 baicalein이 1O2혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호하는데 있어서 ascorbate와 협동함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리킨다.

Keywords

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