Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Purification of Anticancer Peptides from Korean Ginseng

Kim, Sun-Ho;Kim, Ji-Yeon;Park, Sung-Won;Lee, Ki-Won;Kim, Kyoung-Heon;Lee, Hyong-Joo

  • Published : 20030200
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Abstract

To investigate anticancer activity of ginseng, peptides were isolated from Panax ginseng C. A. Meyer, and analyzed for their anticancer activity. Water extract of ginseng powder was fractionated by hydrophobic XAD-2 column chromatography using 100% ethanol as an eluant. The hydrophobic fraction was found to show high anticancer activity on cell line (P388D1, a mouse lymphoma) with an $ IC_{50}\;of\; 13.3\;{\mu}g/mL.$ According to amino acid analysis and sequencing result, the anticancer peptides were composed of tryptophan with a sequence of either trp-trp or trp-trp-trp. The synthetic tripeptide, Trp-Trp-Trp, had the highest anticancer activity on P388D1 cell, whereas Trp in free amino acid form exhibited no effects on the cell. $IC_{50}$ values of dipeptide and tripeptide were 442.9 and $184.9\;{\mu}g/mL$, respectively. In the DNA cell cycle analysis of the tumor cell treated with the peptide fraction by flow cytometry, the DNA contents of S and G2/M phases in the cell cycle decreased, whereas that of G0/G1 phases increased.

Keywords

References

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