Journal of the Korean Orthopaedic Association (대한정형외과학회지)

Korean Orthopaedic Association (대한정형외과학회)
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Effects of Steroid and Nonsteroidal Anti-inflammatory Drugs on Acute Wound Healing and Collagen Synthesis in Rat Skin

백서 피부에서 스테로이드 및 비스테로이드 소염진통제가 급성 창상 치유 및 콜라겐 형성에 미치는 영향

Han, Chang-Dong;Lee, Whan-Mo;Lee, Hun-Bum;Hahn, Myung-Hoon;Yu, Whan-Wook
한창동;이환모;이훈범;한명훈;유환욱

  • Published : 20030000
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Abstract

Purpose : We studied short-term effects of steroid and anti-inflammatory drugs on collagen synthesis in rat skin. Materials and Methods : The 18 rats were divided equally into three groups: group I; piroxicam, group II; triamcinolone, group III; saline. All drugs were given by intraperitoneal injection. Drugs were administered to rats for 4 days after dorsal skin incision. One day after operation and at 24 hour interval there after, skin tissues containing subcutaneous fat were randomly harvested. Measurement procedures were composed of three steps: the first was Masson’ trichrome stain for collagen tissue identification; the second involved transporting the image to a computer and the third the analyses of the collagen area using a morphometry program. Results : Statistically significant differences were found in the mean values of the relative collagen contents of the triamcinolone and saline injection groups on the third day (p<0.05). Relative collagen content of the saline injection group was greater than those of the other groups. The triamcinolone group showed the least collagen content. Collagen content of groups increased in time. Conclusion : Steroid had an inhibitory effect on the synthesis of collagen. Anti-inflammatory drugs also reduced the amount of collagen synthesis, but without statistical difference.

목 적 : 쥐에서 콜라겐의 생성에 대한 비스테로이드 소염진통제 및 스테로이드의 단기 효과를 실험하였다. 대상 및 방법 : 18마리의 쥐를 대상으로 6마리씩 3개의 군으로 나누어 배부에 창상을 만들고 4일동안 복강내 주사로 약물을 투여하였다. 조직 채취는 수술 후 1일 후부터 24시간 간격으로 피부조직을 피하 지방층을 포함하여 무작위로 채취하였다. Masson’s trichrome염색을 이용하여 콜라겐 조직으로 염색된 부분의 사진을 얻고 스캐너를 이용하여 컴퓨터로 영상을 옮긴 후, Morphometry program을 이용하여 염색된 부분의 면적을 측정하였다. 결과 : 날짜에 따른 콜라겐의 상대적 측정치의 차이는 3일째에 스테로이드군과 생리식염수군에서 통계적으로 유의하게 나타났다(p<0.05). 실험기간 동안 콜라겐의 상대적 측정치는 생리식염수 군이 제일 높았으며, 이후 piroxicam군, triamcinolone군의 순서였다. 세 군 모두에서 콜라겐의 양은 시간이 지남에 따라 증가하는 양상을 보였다. 결론 : 스테로이드는 비스테로이드 소염진통제와 대조군에 비하여 콜라겐 합성을 억제하는 것으로 나타났다. 비스테로이드 소염진통제는 대조군에 비하여 콜라겐의 생성량에 감소를 보였으나 의미 있는 차이는 발견되지 않았다.

Keywords

References

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